Priyanka Singla Email & Phone Number

Vancouver, Washington, United States

Portland, Oregon, United States

• Led lab’s patient derived organoid program- designed the study and performed the downstream assays, including metabolomics and single-cell RNA-sequencing.• Performed QC and downstream scRNA sequencing analysis.• Analysed other data like cytokine assay data and bulk-RNA sequencing data.• Managed the lab and mentored the new lab members.

Portland, Oregon, United States

• Grow and maintain cancer cell lines and perform drug proliferation, cell viability assays like CTG, triplex assay, flow cytometry and other assays like qPCR, western blot • Grow organoids from tumor biopsies and perform various assays on them including DNA sequencing, bulk RNA sequencing, FFPE staining and imaging, • Explore and analyze various cancer and drug sensitivity databases like DepMap, PRISM, CTRP, R2- volcano plots, PCoAs, UMAPs, distance matrices, drug response curves using R

Corvallis, Oregon, United States

• Learned to handle several mouse lines and various mouse models including the Maternal Immune Activation model in C57BL/6 mice. My skills include breeding and colony management, behavioral assessment methods like marble burying, open field, elevated plus maze, and three‑chambers sociability assay and more complex tests like forced swimming tests, anesthesia and euthanasia, and necropsy/dissection techniques including brain dissections• Genotyping of mice from ear and hair samples.• Consulted with different laboratories to design, pilot, and troubleshoot the protocol for dissociation of mice intestinal cells, isolate and sort GFP‑labelled cells by Fluorescent activated cell sorting and magnetic sorting, and culture them.• Worked with a neuroscientist to culture the nodose neurons, co‑cultured them with enteroendocrine cells to check the synapse formation.• Performed anaerobic culturing of bacteria and various cell viability assays.• Gained experience working with dissecting scope, compound microscope, epifluorescent microscope, and confocal microscope.• Generated protocols and maintained records of different versions to make sure other lab personnel could follow and understand protocols for cell culture.• Performed immunohistochemistry and flow cytometry to study different gut epithelial cells• Proficient in skills like extraction of genomic DNA, RNA extraction, quantification, PCR amplification, and library prep.• Acquired skills in processing, analyzing, and visualizing 16S sequencing data using R.• Performed comparative and phylogenetic analysis of bacterial genome: used databases like IMG and PATRIC to find unique KEGG pathways, pathogenic genes and create phyloseq objects, ordination plots and phylogenetic trees using R.• Analyzed mice behavior data using R.• Learned to use the command line (UNIX/LINUX) to run BLAST and 16S community sequence analysis using MOTHUR.• I have also been exposed to the methodology and concept of electrophysiology

Corvallis, Oregon, United States

• Online teacher for BI213 (Principles of Biology Lab)- lectured 40 students about the week’s lab, designed and graded the quizzes/assignments, and assisted them in the lab activities.• Teaching assistant for MB311 (Molecular Microbiology Lab)- assisted 50 students with multiple molecular biology techniques like plasmid isolation, PCR purification, electrophoresis, transformation, CRISPR-Cas9 genome editing, and 16S community bioinformatics analyses• Teaching assistant for MB230 (Introductory Microbiology Lab) and MB303 (General Microbiology Lab)

Sahibzada Ajit Singh Nagar, Punjab, India

Worked on a project “Full characterization of 3 probiotic bacteria: Lactobacillus plantarum,Lactobacillus reuteri, Lactobacillus casei, and formulation of synbiotic drink using these 3bacteria and studying its antimicrobial activity.”• Performed various biochemical tests and sugar fermentation tests on three probiotic strains• Studied other characteristics like thermal point, hydrophobicity index, acid and bile tolerance and antimicrobial activity• Prepared a synbiotic drink and checked it's stability over time

Bathinda, Punjab, India

• Taught courses‑ Biomedical Signal Processing, Environmental Science, Human Values and Professional Ethics• Involved in student counseling services and conducted a survey on how students feel about deforestation

Bathinda, Punjab, India

• Taught courses‑ Biomedical Signal Processing and Environmental Science• Successfully organised workshop on 'Basic Robotics', organised guest lectures on "Forest Conservation Education", "Sustainable power generation", participated in Job Fair of 2013

Master'S Degree, Microbiology, General, Gpa Of 3.85

Bachelor'S Degree, Biotechnology, Cgpa Of 8.5(On Scale Of 10)

Activities and Societies: • Certified as a member of the Arts Committee of Jaypee Youth Club, Waknaghat. • Active member of the Biological.