Pruthvi Chari, Ph.D. Email and Phone Number

Analytical Scientist- responsibilities included developing and validating analytical methods for small molecules using HPLC/UPLC (Waters), GC (PerkinElmer), and PSD (Mastersizer3000). Additional Job responsibilities included authoring/reviewing test methods, validation protocols, validation reports, GMP documentation and review of GMP lab notebooks, providing technical updates, presenting technical data during teleconferences, training new hires, handling controlled substances, technical data analysis, and interpreting.

Biophysical characterization of select proteins to understand charge-charge interactions using various spectroscopic techniques viz fluorescence, CD, and AUC. Job responsibilities included carrying out projects independently and through collaboration, involving experimental work, data analysis, data interpretation, technical presentations, and technical writing. Additional responsibilities were to guide/mentor undergraduate students in their research projects, and maintaining/ordering laboratory supplies.

Functional analysis of AdoMet binding residues in yeast Sterol Methyltransferase enzyme by site-specific mutations and kinetic analysis.

Functional and thermodynamic analysis of yeast sterol methyltransferase by selective mutagenesis, kinetics, and photo-affinity labeling studies.

Biophysical studies on a candidate protein using spectroscopic techniques such as fluorescence and circular dichroism.