=> Techniques d'enseignement : conception, réalisation et évaluation de séquences d'enseignements et de projets pédagogiques pluridisciplinaires, élaboration et adaptation d'échéanciers en lien avec des objectifs et imprévus, management humain individuel et collectif (gestion de l'hétérogénéité), gestion de projet de développement durable (coordination du comité de pilotage du Lycée Eco-Responsable : blog, cantine "zéro gaspi", compostage, apiculture), apprentissage par méthodologie scientifique (hypothèses/expériences/résultats).* Objectifs : Assurer l'assimilation des connaissances en vue de la réussite au Bac. Développement des compétences disciplinaires, méthodologiques et sociales. Accompagner les élèves dans leur développement humain et citoyen. Transmettre une méthode rigoureuse d'analyse critique. Les sensibiliser au changement de société à venir en terme de protection de l'environnement et de la santé des populations.

Manager of partnerships and projects. Member of the Board (200K€ of annual budget, 1000 members, more than 120 actions per year). For 30 years HCE organizes mountain walks for disabled people (www.hce-asso.fr).• Prospecting, negotiations and follow-up of new partnerships and networks.• Human and logistic management. Sustainability and valorisation of activities.• Recruitment by communication tools (flyers, social networks, web reporting)• Representative (partners, local media) and coordinator (for the 15 local branches)

=> Sociological techniques : Lead interviews, ethnographic description (Laplantine, 1996, 2015), analytical induction (Kaufmann, 2011).Sociological analysis of issues and relationships between Expert and Layman about molecular oncology (National Health Innovations Days and Centre Léon Bérard : C. Bourgain, D. Cox, X. Guchet, L. Eberst, J. Vailly, J. Henry)• Interviews with different actors of molecular oncology network (patients, oncologists, physicians, scientists, sociologists, officials). In-depth analysis of socio-economic issues and needs raised (14 interviews, 6 field observations, 1 published article and 1 in modification).Co-founder of a plateform wich share professional skills about prevention and screening of chronic deseases (www.prominystia.org). (Medical and scientific network : D. Cox, P-E Heudel, J-Y Blay, O. Tredant).• Monthly meetings, public talks, website publications, scientific and public press watch, CLARA congress and network.• Reflexions on the development of the 4P medicine.

=> Biomolecular techniques : cell cultures, siRNA transfection, RNA extraction, RT-qPCR, PCR, microarray HT12 (illumina), in silico miRNA targets research, bioinformatics, agarose electrophoresis, confocal microscopy. * Main goal : perform a set of experiments for identifying a new in vitro metastasis biomarker (potential target genes of the microRNA hsa-miR500a).Epigenetic group, Z. Herceg labteam : microRNA and Breast Cancer

=> Biomolecular techniques : cell culture (HeLa), WB, Luciferase Assays, RNA extraction, RT-qPCR, PCR, siRNA transfection, agarose electrophoresis, Bradford protein assay. * Main goal : perform final sets of experiments allowing the publication of a paper about the influence of Fibrillarin Knock-Down (siRNA) on the translation initiation (Cap- or IRES-dependent).In Diaz Labteam : Work on ribosomal RNA methylation in tumor cells.

=> Radiobiological techniques : cell culture on primary human tumor cell, immunoprecipitation, kinase tests, nuclear protein extraction, Bradford protein assay, cell irradiation, immunofluorescence, confocal microscopie, WB.=> Collaboration with the Neolys diagnostics company for the development of a radiosensitivity test. * Main goal : demonstrate the nucleoshuttling of the ATM protein in response of DNA double-strand breaks induced by therapeutic radiation doses. Radiobiology (N. Foray labteam)

Histological and laboratory animal house techniques : WB, NB, DNA extractions, RT- PCR, cell culture, preparation FFPE and staining of histological samples for Optical Microscopie observations (Halun tetrachrome, HES, Masson trichrome, Raifort’s Peroxydase). Preparation of histological samples (metallization) for TEM and SEM observations. Animal handling (weighing, feeding, subcutaneous treatment, observation of stereotactic microsurgery)Main goal : Histological staining of pituitary samples as diagnostic and prognostic markers in pituitary tumors. Histology of the pituitary cancer - Oncoflam (Honnorat labteam)

Techniques de vente, conseil client, objectif de résultats.