Internship missions : Development of an innovative and scalable purification process of recombinant baculoviral vectors for in vivo gene delivery. Lab activity : The goal of the unit U1179 of neuromuscular diseases and biotherapies laboratory is to develop strategies for splicing modulation (exon skipping), genome editing and gene delivery for therapeutic application, using both viral vectors (AAV, lentivirus, HSV) and antisens oligonucleotides (TcDNA technology). All these… Show more Internship missions : Development of an innovative and scalable purification process of recombinant baculoviral vectors for in vivo gene delivery. Lab activity : The goal of the unit U1179 of neuromuscular diseases and biotherapies laboratory is to develop strategies for splicing modulation (exon skipping), genome editing and gene delivery for therapeutic application, using both viral vectors (AAV, lentivirus, HSV) and antisens oligonucleotides (TcDNA technology). All these strategies aim for re-establishment of dysfonctional protein activity involved in genetic diseases as neuromuscular disorders (DMD, SMA, ...). Tools and technics : - Work in containment level 2 & 3 laboratories- Cellular : cell culture, virus production in insect cells (BEVS System), transductions, flow cytometer (initiation)- Biotech : tangential flow filtration (TFF), isopycnic ultracentrifugation, diafiltration (amicon®)- Molecular : SDS-PAGE, silver staining, QPCR Titration, DNA extraction. Show less

Groupe MICALIS (Microbiologie de l’Alimentation au service de la Santé)Team ProCeD (Dr.Carballido-López)Subject : INTERACTION OF AMINOGLYCOSIDES WITH ENTEROCOCCUS CELL WALLBACKGROUNDEnterococci have an innate resitance to aminoglycosides that is independent of the presence of any enzymes that may inactivate the antibiotic or any modifications of the ribosomes that would render the interaction with antibiotics less avid. This innate resistance is often explained by the… Show more Groupe MICALIS (Microbiologie de l’Alimentation au service de la Santé)Team ProCeD (Dr.Carballido-López)Subject : INTERACTION OF AMINOGLYCOSIDES WITH ENTEROCOCCUS CELL WALLBACKGROUNDEnterococci have an innate resitance to aminoglycosides that is independent of the presence of any enzymes that may inactivate the antibiotic or any modifications of the ribosomes that would render the interaction with antibiotics less avid. This innate resistance is often explained by the incapacity of aminoglycosides to enter the enterococcal cell.Our preliminary results indicate that the aminoglycosides interact with enterococcal cell wall with an affinity one order of magnitude higher than with cell walls of Bacillus subtilis and Staphilococcus aureus. We hypothesize that this interaction may explain the innate resistance of Enterococci to aminoglycosides.Enterococcus faecalis is a very diverse genus with differing strains displaying a wide structural and chemical variety of secondary polymers on the cell wall.PROJECTWe want to test whether the variety of cell walls translates in differing innate resistances to aminoglycosides. We have obtained a dozen strains of Enterococcus faecalis from the collection of Pascale Serror (1/3 clinical isolates, 1/3 isolated as food contaminants, and 1/3 intestinal commensals). We have tested ten strains and identified a strain whose innate resistance to aminoglycosides : JH2-2.We are going to test whether these differences in innate resistance to aminoglycosides correlate with differences in the affinities of the aminoglycosides with the cell walls of these strains. 1) Purification of Enteroccal strains cell walls.2) Affinity measure of aminoglycosides on cell wall by microcalorimetric study [Not achieved] Show less