Purification of Peptides.

Working on Developmental scale (R&D) responsible for process development for protein purification and Tech Transfer. Handling various chromatographic operations, Handling AKTA Process system (Basic, Explorer, Pure Avant, Pilot),Handling and operation of TFF, Running SDS gel, Handling UV spectrophotometer, Osmometer, Turbidimeter.

Involved in Downstream Processing of Zycov-D (Novel Corono Virus 2019-nCov Vaccine Drug Substance),Working on TFF (Tangential Flow Filtration) System of Sartorius make, Cassette Area 105m² EFA (one of the largest Area system) and performing concentration, Diafilteration and recovery of the product,NWP (Normalised Water Permeability) calculations, Handling of various chromatography operation,Handling Pallotronic 4 and Sartorius 5 Integrity machine,cGMP Documentation. Handling QMS Documents such as Deviation, Change control and CAPA(Corrective Action and Preventive Action),Preparation of SOP, BMR, MFR.

Excepient Solution Prepation, Final Vaccine Formulation(viral(Rotavac) and Bacterial (Cholera)),Handling SCADA enabled vessels, Expertise in Filter Integrity Testing, Handling SAP software, SOP preparation, Involved in Qualification of Mixing vessels, Equipment installation and qualification in accordance with manufacturing sites, QMS - Handling inprocess deviations, change control and CAPA ( Corrective action and Preventive action)

Monoclonal Antibody Purification (on AKTA process) Using Affinity, Anion Ex change ,cation Ex change Chromatography Techniques and Tangential Flow Filteration(TFF) -DownStreamProcessing

Screening of Bacterial Blight Resistance Genes in GK Paddy Inbred Lines unsing Molecular Markers.