Rajesh Kishore Kumar Sanku, Ph.D. Email and Phone Number
Rajesh Kishore Kumar Sanku, Ph.D. work email
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Rajesh Kishore Kumar Sanku, Ph.D. personal email
A passionate and highly motivated scientist with a strong background in protein biochemistry, and molecular biology. I have over 6 years of experience in biochemistry, PK-PD (Pharmacokinetic/Pharmacodynamic) profiling of drugs and modern bioanalytical research.• Developing bioanalytical methods for quantitation and metabolism/biotransformation assessment of small molecule drugs, antibodies, fusion proteins, antibody drug conjugates and Probodies•PK Exposure using ELISA/LBA and Biologics quantitation using MSD. •Translational Biomarker studies on clinical samples.•Proteomics for development of qualitative/quantitative assays for proteins/peptides and post-translational modifications.•Metabolomics on high-resolution Orbitraps and Triple Quadrupole MS.•LC-MS sample preparation from biological matrices (cell, tissue, serum, plasma).Technical skills: Protein expression and purification, stable isotope labelling with amino acids in cell culture(SILAC), immunoprecipitation, parallel reaction monitoring(PRM), selected reaction monitoring(SRM), multiple reaction monitoring(MRM), solid phase extraction(SPE), liquid-liquid extraction(LLE), lipid extraction, BCA assay, Immunoassays, gel-electrophoresis, cell cultures(2D, 3D), neurotoxicity, western blotting, microsomal stability, plasma protein binding, permeability assays, metabolite identification, solubility, lipophilicity and enzyme kinetic studies. UPLC, HPLC, 2D-nano-UPLC(Dionex), Thermo Q Exactive HF Hybrid Quadrupole-Orbitrap, TSQ triple quadrupole, Waters TQ MS. Mass spectrometer maintenance including removing/cleaning/inserting of parts like ion transfertube/tube lens/skimmer/pump/Q0 and regular tuning and calibration of the instruments.Interpreting mass spectrometry data and statistical analysis using tools Xcalibur, Tracefinder, Mass Hunter, MassLynx, TargetLynx, Skyline, proteome discoverer, GraphPad Prism.►Connect with me on LinkedIn or email me directly-rajeshkishorekumar@gmail.com.
Exelixis
View- Website:
- exelixis.com
- Employees:
- 51
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Senior Scientist 2Exelixis Nov 2024 - PresentAlameda, California, Us
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Reviewer Of Bioorganic & Medicinal Chemistry LettersElsevier Publishing Services Apr 2019 - Present -
Drug Metabolism And Pharmacokinetics (Dmpk) ScientistGilead Sciences Jan 2021 - Oct 2024Foster City, Ca, Uso Developed and validated bioanalytical LC-MS/MS methods for the quantitative and qualitative analysis of peptides, proteins, antibodies, and antibody-drug conjugates (ADCs). Designed PK/PD and ADA assays, and conducted sample analysis, including non-compartmental analysis (NCA) of LC-MS and MSD data.o Collaborated with Contract Research Organizations (CROs), overseeing outsourced bioanalytical projects to ensure alignment with project goals and timelines.o Assisted in the preparation and authoring of regulatory documents for nonclinical PK assessments, including First-in-Human (FIH) dose projections.o Represented projects independently in core team meetings, presenting and interpreting results, and authoring technical reports and presentations.o Utilized bioanalytical assay expertise to drive key product development decisions, including translational PK/efficacy modeling, ensuring regulatory compliance, and managing CRO activities. -
Research Scientist 2Charles River Laboratories Mar 2020 - Jan 2021Wilmington, Massachusetts, Uso Worked for various clients in the discovery bioanalysis team for method development, implementation, and validation of various bioanalytical assays using PK/PD ligand binding assays/ELISA, MSD of macromolecules.o Transferred the validated method to research associates for the high throughput screening.o Developed bioanalytical LC-MS/MS methods for small molecules using various mass specs. -
ScientistBristol-Myers Squibb Jun 2019 - Mar 2020Lawrence Township, Nj, Uso Developed bioanalytical LC-MS/MS methods for the quantitative and qualitative analysis of peptides, proteins, antibodies and antibody-drug conjugates (ADCs) in biological sampleso Sample preparation and analysis of biotherapeutics from PK samples including immunoprecipitation/affinity capture using bead-based and/or automated platformso Quantitative method development of biologics: Peptide map, identification, and optimization of appropriate MRMs using Skyline software and LC-MS instrumentation (Triple Quadrupole, TOF, Orbitrap)o Independently performed complex experiments with strong attention to detail and documentation.o Performed In vitro serum stability assessment of ADCs using mono affinity and dual affinity capture methods and analyzed their biotransformation and metabolites using LC-HRMS. o Contracting, managing and overseeing outsourced bioanalytical and ADME activities for both early Translational and Non-Clinical Development programs -
Postdoctoral ResearcherUniversity Of Pennsylvania School Of Medicine Jun 2018 - Jun 2019Pa, UsWorked on a 2 Million dollars project which aims at the Biomarker identification of early cancer detection.• Performed assay development for sample preparation protocols from biological matrices for mass spectrometry analysis; establish SOPs.• Development and validation of quantitative methods for large and small molecule analysis using nano-LC and mass spectrometry. • Implementation of various mass spectrometry methods, for proteomics, lipidomics and metabolomics.• Experience with protein post-translational modification analyses.• Determination of small molecule metabolites (e.g. steroids) using chemical derivatization from biological matrices related to cancers. -
Phd ResearcherTemple University Sep 2013 - Apr 2018Philadelphia, Pennsylvania, UsMy research was focused on PK/PD screening of Anti-cancer and CNS drugs.As a doctoral candidate I:•Worked under two advisors (biochemist and behavioral pharmacologist) on a collaborate dissertation project. •Developed protocols, designed experiments, collected and analyzed data which resulted in presentations at 8 national conferences, 2 manuscripts and 1 book chapter.•Maintained collaborative relationships with 3 research groups to support research goals.•Developed various LC/MS/MS techniques and executed experiments, such as microsomal stability, blood brain barrier permeability, plasma protein binding solubility, lipophilicity, toxicity assays, to screen novel drugs for their optimal pharmacokinetic properties. •Designed and carried a metabolomics study, analzed and integrated protein expression and cell metabolosim data to identify to find a potential biological pathways leading to discovery of novel biomarkers during a collaboration with University of Pennsylvania. •Set up and performed various rodent behavioural assays: autoshaping task, novel object recognition test (NORT), novel object location test (NLRT) and drug discrimination to investigate mechanism of action of various novel CNS drugs•Trained 17 graduate students and 2 undergraduate students with their research projects which lead to poster presentations at Temple University and other national conferences. -
Pharmacovigilance ScientistNovartis Jan 2012 - Jun 2013Basel, Baselstadt, Ch• Worked on ARGUS, safety database management software used for reporting to various regulatory authorities like F.D.A, Medicines and Healthcare products Regulatory Agency. -
Summer InternHetero Drugs Ltd May 2010 - Jul 2010Hyderabad, In• Worked with Bioequivalence testing department.• Assisted with coordination, preparation and review of clinical trial applications.• Responsible for filing applications, follow ups and obtaining permissions/licences for drug approval.
Rajesh Kishore Kumar Sanku, Ph.D. Skills
Rajesh Kishore Kumar Sanku, Ph.D. Education Details
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University Of Pennsylvania School Of MedicinePost Doc -
Temple UniversityPharmaceutical Sciences
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Birla Institute Of Technology And Science, PilaniBachelor Of Pharmacy (B.Pharm.)
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University Of FloridaModel-Informed Drug Development
Frequently Asked Questions about Rajesh Kishore Kumar Sanku, Ph.D.
What company does Rajesh Kishore Kumar Sanku, Ph.D. work for?
Rajesh Kishore Kumar Sanku, Ph.D. works for Exelixis
What is Rajesh Kishore Kumar Sanku, Ph.D.'s role at the current company?
Rajesh Kishore Kumar Sanku, Ph.D.'s current role is Senior Scientist 2 at Exelixis.
What is Rajesh Kishore Kumar Sanku, Ph.D.'s email address?
Rajesh Kishore Kumar Sanku, Ph.D.'s email address is ra****@****bms.com
What schools did Rajesh Kishore Kumar Sanku, Ph.D. attend?
Rajesh Kishore Kumar Sanku, Ph.D. attended University Of Pennsylvania School Of Medicine, Temple University, Birla Institute Of Technology And Science, Pilani, University Of Florida.
What skills is Rajesh Kishore Kumar Sanku, Ph.D. known for?
Rajesh Kishore Kumar Sanku, Ph.D. has skills like Research, Hplc, Lc Ms, Confocal Microscopy, Behavioral Pharmacology, Microtome, Immunohistochemistry, Microscopy, Pharmacovigilance, Argus Safety, Formulation, In Vitro.
Who are Rajesh Kishore Kumar Sanku, Ph.D.'s colleagues?
Rajesh Kishore Kumar Sanku, Ph.D.'s colleagues are Zheny L., Karina Ma, Stephanie Kopp, Andrea Spencer, Akash Das, Phd, Debola Banerjee, John Scott.
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