The development and optimization of a purification strategy using multiple chromatographic methods to scale from milligram to gram quantities for assays, research, and other studies are based on client needs.

Worked on the purification of insulin biosimilars, particularly Glargine. The process was optimized using various chromatographic and non-chromatographic methods

»Downstream processing of human blood plasma proteins by Chromatography. »Lyophilization and cycle development for human plasma proteins- Thrombin, Fibrinogen, FVIII»Proteins samples analysis by SDS PAGE and Western Blot. Total protein estimation by Biuret and Lowery methods.»Microfiltration- TFF »Projects involved: Thrombin, Fibrinogen, Albumin, IgG, and FVIII »Skilled in usage of Akta purifier, TFF, Spectrophotometer, pH meter, Conductivity meter, pipettes, analytical… Show more »Downstream processing of human blood plasma proteins by Chromatography. »Lyophilization and cycle development for human plasma proteins- Thrombin, Fibrinogen, FVIII»Proteins samples analysis by SDS PAGE and Western Blot. Total protein estimation by Biuret and Lowery methods.»Microfiltration- TFF »Projects involved: Thrombin, Fibrinogen, Albumin, IgG, and FVIII »Skilled in usage of Akta purifier, TFF, Spectrophotometer, pH meter, Conductivity meter, pipettes, analytical weighing balance »Technology Transfer for liquid and Lyophilized products Show less