Biological Engineer l RNA Design and Biosynthesis l Proteins l Small-Molecules l Cell-Free Biosynthesis• 12+ Years of research experiences including industrial research in the field of microbial synthetic biology, bioengineering, biochemistry of natural products, protein, and nucleic acid (RNAs, DNA) biosynthesis.• Excellent project management, leadership, teamwork, and problem-solving skills• Registered-IPs• Peer-reviewed scientific research and review articles, book chapters published in leading scientific journals• Editorial Board Member I Reviewer of numerous Biotechnology journals• Scientific Project Development and ManagementIn sum, the technical expertise includes, but not limited to:• Cell-Free Reactions (CFR) for biosynthesis of natural/non-natural small/large molecules such as RNAs (mRNA, cirRNA, long and short dsRNA)• Nucleic acid biochemistry (Biosynthesis of precursor molecules [NMPs, NDPs, NTPs, and modified nucleotides], pDNA template design for IVT, RNA design and production using IVT, purification and analysis, and functional characterization using in vitro/ in vivo reporter assays)• Genome mining for novel enzymes, difficult to express enzymes production, purification, analysis, and protein biochemistry (enzyme kinetics, assay development, end-to-end cell free reactions)• Microbial engineering for biosynthetic pathway design, fine-tuning of the biosynthetic pathways (combinatorial pathway design) using state-of-the- arts synthetic biology and metabolic engineering tools in E. coli, Streptomyces, and yeast• Metabolites analysis using various H/UPLC, Prep–HPLC, LC–MS, and NMRs. Personal Links:Google Scholar: https://scholar.google.com/citations?user=7HMWnYAAAAAJ&hl=en&oi=ao
New Co.
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Senior ScientistNew Co. Jul 2023 - PresentCambridge, Massachusetts, United States
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Scientist IiGreenlight Biosciences, Inc. May 2021 - Jul 2023Boston, Massachusetts, United StatesProject leading to discover:• A super high copy plasmid backbone for template pDNA production used for IVT• Various architectures of RNA (mRNA, cirRNA, cap and tail free RNAs) for protein expression in animals and plants• Biosynthesis of D-Allulose, a low calorie sugar molecule using Cell-Free Reactions starting from maltodextrin The discoveries are expected to be developed as potential IPs.Achievements:• Led to develop a cost effective end-to-end cell-free biosynthetic route to produce a molecule from cheap resources; reduced the cost of molecule by 70% while increasing the yield by several folds.• Developed a novel platform to produce long dsRNA with improved yield/titer with superior purity, reducing the overall cost of the production.• Filed Tech Disclosures: submitted ideas to the business development team for production of for commercially important molecules via novel biosynthetic routes.Project Management:• Project development and management: Project ideation, planning, cost management, and timeline projection• Brainstorming: Actively participating in brainstorming session and providing feedback to the upper management team• Delivery of outcomes among stakeholders/different departments, teams, and townhalls within the organization• Team building: Mentorship to scientists and associates -
Postdoctoral Research AssociateMassachusetts Institute Of Technology Mar 2020 - May 2021Cambridge, Massachusetts, United States• Designed retrosynthetic non–natural pathways for biosynthesis of precursors of high–value molecules in microbes I Project Lead• Design the project work and execute experiments in the lab • Used state–of–the–arts synthetic biology and enzyme catalysis techniques and tools -
Postdoctoral Research AssociateBroad Institute Of Mit And Harvard Mar 2020 - May 2021Cambridge, Massachusetts, United States -
Research Assistant ProfessorSun Moon University Mar 2016 - Feb 2020Asan, Chungcheongnam-Do, Korea• Genome mining and discovery of highly promiscuous glycosyltransferases enzymes from Bacillus for biosynthesis for O–, N–, and S–linked glycorandomized medicinally important natural and synthetic molecules (Organic Letters 2019, 21, 8058–8064)• Discovery of polyketide–based anthraquinone biosynthetic route in Senna tora plant using genomic, transcriptomic and metabolomic analyses. (Nature Communications 2020)• Developed a One–Pot Multienzyme Cofactors Recycling (OPME–CR) system for glycodiversified plant and microbial secondary metabolites• Developed microbial strains for regiospecific biotransformation of molecules to generate non–natural small molecules chemical library • Enzyme engineering for substrate promiscuity and regiospecific modification of substrates• Identified and functionally characterized pathway enzymes involved in Nargenicin A1 biosynthesis in Nocardia (ACS Chemical Biology 2020, 15, 1370–1380) -
Research ProfessorSun Moon University Sep 2014 - Feb 2016South Korea• Developed enzymatic and microbial biosynthesis approaches for production of hydroxylated derivatives of flavonoids using CYP450 BM3 variants (Microbial Cell Fact. 2016, 15, 135)• Designed synthetic/artificial biosynthetic routes for enzymatic synthesis of novel diverse sugars conjugated non–natural molecules• Microbial fermentation (5–L volume), and preparative scale enzyme reaction (1–L scale) for production of target molecules -
Research FellowSun Moon University Mar 2010 - Aug 2014Focused on development of E. coli platform strains for enzymatic and microbial biosynthesis of plant metabolites• Worked as a team leader, trained and supervised undergraduate and graduate students resulting multiple first authored and co–first authored articles in peer–reviewed research journals published by American Society for Microbiology (AEM), Biotechnology and Bioengineering, Applied Microbiology and Biotechnology• Developed E. coli strains by genome editing to redirect central carbon metabolism towards target molecules.• Engineered plant flavonoids biosynthetic pathways in E. coli and optimized biosynthesis parameters, scaled–up biosynthesis using lab scale fermentation• Developed enzymatic biosynthesis approaches for production of diverse natural products glucosides
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Visiting ScientistEth Zurich Jul 2018 - Aug 2018Zürich, Switzerland• Studied epoxide ring formation in herboxidiene, a type I polyketide molecule produced by Streptomyces chromofuscus• Functional characterization and substrate promiscuity of type III polyketide synthase enzymes towards diverse starter CoAs -
Research TraineeMolecular Biology Unit, National Dairy Research Institute Jan 2009 - Aug 2009Karnal, India• Cloning of 'Mucin Binding Protein' from Lactobacillus plantarum LP-91• Isolation of pure culture of different Lactobacilli (~200) from ~20 different stool samples• Isolation of Lactobacilli and Bifidobacteria from mother's colostrum • Isolation of total DNA from ~20 stool samples• Handling of mice
Ramesh Pandey Education Details
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Molecular Biology -
Microbiology, General -
Tribhuvan UniversityMicrobiology, General
Frequently Asked Questions about Ramesh Pandey
What company does Ramesh Pandey work for?
Ramesh Pandey works for New Co.
What is Ramesh Pandey's role at the current company?
Ramesh Pandey's current role is Developing Next-Gen Molecules for Humanity l PhD l MIT Postdoc.
What schools did Ramesh Pandey attend?
Ramesh Pandey attended Sun Moon University, National Dairy Research Institute, Karnal, Kurukshetra University, Tribhuvan University.
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Ramesh Pandey
Unleashing Ai’S Potential: Transforming Health Data Into Insightful Narratives @ Health And Life Sciences Data Platform, MicrosoftRedmond, Wa1microsoft.com -
Ramesh Pandey
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4yahoo.com, allergan.com, abbvie.com, maynepharma.com
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Ramesh Pandey
Millburn, Nj2yahoo.com, chubb.com6 +414321XXXXX
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