Technical biology lead for a Global New product introduction (NPI) providing expertise and leading multi-site developmental efforts in CAR-T and TCR processess across a multi-disciplinary group. Also, managing and mentoring technical team members.

 I have focused on building and leading process development initiatives for large-scale expansion of T cells and NK cells in Xuri W25 rocking bioreactor and stirred tank reactors. GE Enterprise Flex Factory Client Project (Asia Pacific Client) - 2018-2020 o Role: Technical Leado Unit Operations(CliniMACS Plus, Sepax, Xuri W25, Sefia, Dynamag, VIA Freeze, CryoMed, VIA Thaw).o Developed a closed and semi-automated manufacturing process of TCR cells.o Optimized upstream and downstream culture process at 1L, 5L scale.o Characterized initial and final cell product using cell based assays. Re-designed and developed a universal T Cell panel - 2019-2020o Role: Lead (Unit operations: Cytoflex, BD Lyric, BD Canto, BD Fortessa)o Developing a surrgate T cell panel with GFP transduced and non-transduced T cells. GE New Product Introductions (NPI): Media Formulation Team - 2019-2020o Role: Technical Lead (Unit operations: Sepax, Xuri W25 Rocking Bioreactor, Cytoflex).o Small scale and Large scale evaluation of new media formulations with different culture systems.o Developed URS, Plan V&V, Media CMO intereactions to assess batch variability. Disruptive Technologies: - 2018o Role: Co-lead (Unit operations: Xuri W25 Rocking Bioreactor, Eppendorf Dasbox STR, Cytoflex)o Hand built and tested a pre-designed prototype bioreactor with CHO, T cells and PSC.o Compared the performance of the prototype with commercially available bioreactor platforms.o Reported product design recommendations to the engineering team. Large-scale expansion of Natural Killer (NK) Cells: - 2016-2017o Role: Co-lead (Unit operations: Sepax, CliniMACS, Xuri W25 rocking bioreactor, Sefia, VIA Freeze, VIA Thaw, Cytoflex, TECAN plate reader and washer).o Developed semi-automated and functionally closed manufactured process.o Characterized cell product using cell based assays such as NK killing assay.o Wrote an application note, abstract and poster for NK manufactured process (ISCT).

 I worked as a Flow Cytometry associate at The Center for Phenogenomics (TCP)/Canadian Mutant Mice Repository (CMMR). I was a member of an exciting research team contributing to the goals of an international consortium which aims at converting Embryonic Stem cells with targeted gene mutations into knockout mice for phenotyping and generating novel Cre driver mouse lines. My main responsibilities include:o Preparing and running 8 color panel BD LSRFortessa II for data acquisition from stained splenocytes.o Data analysis to identify immune cell populations to compare phenotype profile.o Writing report on study conclusion.o Inventory management

 Worked in an oncology lab that focused on testing how immunity develops after hematopoietic cell transplantationo Experience in processing and preserving mononuclear cells from peripheral blood of bone marrow transplant pediatric and adult recipients. o Experience in clinical database management.o Experience in working with FACS ARIA II. o Experience in clinical trial coordination.

 “Analysis of therapeutic proteins derived from recombinant DNA technology for its Identity, Purity and Potency” • Conducted analysis of Erythropoietin that was synthetically produced in mammalian cell cultures to determine its identity, purity and potency• Employed techniques such as SDS-PAGE, Western blot, ELISA, HCP and HPLC and compared it to the required standards of the European pharmacopeia. • Recorded experimental results and performed data analysis

 In-plant training in Process Technology for Recombinant Therapeutics• Exposed to various Protein Purification and Bioprocess Operations• Performed SDS PAGE and carried out silver staining • Prepared Media, Pre-Inoculums, reagents and solutions• Assisted in Induction of Cell culture and Solubilization• Assisted in performing Gas-liquid Chromatography

 Implant training on “Plant tissue culture techniques- Exoecaria agallocha and Jatropha curcas” • Carried out Micropropagation of Jatropha Curcus and Exocoecaria Agallocha• Analysed the Effects of Hormone Balance on Explant Growth and Morphogenesis• Trained in the preparation of various types of growth media • Assisted in Callus induction and Multiplication• Prepared suspension cultures