Dr. Ramya Stehling (Née Nair) Email & Phone Number

Munich, Bavaria, Germany

Munich Area, Germany

• Independent management of research projects in hematology, virology, biochemistry• Close collaboration with international scientists in materials science and immunology• Analytical optimization and troubleshooting of biochemical laboratory experiments• Supervision of 5 bachelor/master laboratory interns (incl. definition of work packages, regular feedback meetings, and review of work results)• Communication of scientific work to professional and lay audiences, including at international conferences (e.g., , Controlled Release Society)• Preparation of Standard Operating Procedures (SOPs) for efficient laboratory procedures• Initiation, supervision, and completion of a branding project to improve visibility of research group (logo & website design)Scientific expertise:• Experience working in a biosafety level 2 environment• Expertise in biochemical methods: Protein purification, western blot, size exclusion chromatography, protein-protein interaction assays• Expertise in cell culture techniques: maintenance of cell lines, isolation of PBMCs and T cells from whole blood, planning, execution, and optimization of high-throughput cellular assays in multi-well formats, genetic modification of cells through transfection and nucleofection• Expertise in nucleic acid techniques: qPCR, PCR, RNA isolation, Gibson assembly cloning, ligation-based cloning, transformation of chemically competent E. coli• Extensive experience in purification of viral vectors and virus-like particles from cell supernatant, quantification of lentiviral RT activity via Real-Time PCR-based assay, transduction of cells, virion fusion assay• Extensive experience in flow cytometry, as well as microscopic analysis of fixed and stained cells

Munich Area, Germany

• Establishment and optimization of the workflow of the biobanking project, from procurement to documentation of biological samples of transplant patients• Onboarding and training of new team members regarding existing processes• Organization of laboratory and consumable materials for easy inventory and supply managementScientific expertise:• Isolation of serum and PBMCs from whole blood

Munich Area, Germany

Thesis title: Functional characterisation of unique Cag Type IV secretion system components CagP and CagG in Helicobacter pyloriExperimental methods: RNA isolation and purification, qPCR, Northern Blot, western blot, PCR and gel electrophoresis, bacterial transformation and plasmid prep, cloning, primer and probe design, sequence analysis

Munich, Bavaria, Germany

• Conception and design of multimedia marketing materials for the event• Identification of marketing distribution channels to reach target audience, resulting in >150 participants• Professional use of graphic design tools such as Adobe Illustrator and Canva• Cross-functional collaboration with colleagues from different teams (content, logistics, finance) for the successful execution of the event

Munich Area, Germany

Title: Characterisation of C-terminal domain mutants of the RNA Polymerase II protein in B-cells (Raji cell line) for mass spectrometry.Experimental methods: Mammalian cell culture and transfection, western blot

Munich Area, Germany

Title: High-throughput screening of a library of compounds against Helicobacter pylori CagA transport via the type IV secretion system into stomach epithelial cellsExperimental methods: High throughput fluorescence-based screening of compounds, bacterial transformation, cloning, western blot, PCR

Thesis Title: Charactisation of a new anti-mycobacterial class of compounds against Mycobacterium tuberculosis, using M. smegmatis and M. bovis as model organisms. Experimental methods: High throughput screening of synthetic compounds, bacterial culture, growth curve generation, dose reponse curves generation, culture of non-replicating models of M. bovis BCG

Germany

Title: Characterisation of an Acinetobacter baumanii transglycosylase mutant to find genes involved in motility.

Dr. Rer. Nat., Hematology And Virology, 1.0

Master’S Degree, Biology/Biological Sciences, General, 1.2

Bachelor Of Science (B.Sc.) (Hons.), Biological And Biomedical Sciences, Cap: 4.2/5.0, Second Class (Upper)

Activities and Societies: Head of Welfare and Project Director, i.CARE Network NUS - event organisation targeted at international students

Exchange Programme, Biology/Biological Sciences, General