Raphael Klein Email & Phone Number

Sankt Augustin, Nordrhein-Westfalen, Deutschland

Neuss

I was responsible for the planning and organisation of R&D projects in the field of immunology.The aim was to development innovative Immunoassays.The work included upstream and downstream developement of recombinant proteins in bacterial and mammalian cells, bioconjugation of allergens and antibodies, validation of allergens by SDS-PAGE, Immuno-Blot and ELISA.

Mainz

During my PhD project I was interested in finding new starting points for the design of antibiotics. 3 proteins served as targets: FabF, an enzyme of the fatty acid synthesis cycle of bacteria and two carbapenemases (GES-5 and KPC-2).I used virtual screening and fragment screening methods. In order to identify hit compounds I developed a binding assay using Bio-Layer-Interferometry and was able to prove the concept of an innovativ enzymatic assay. Hit compounds were co-crystallized in order to determine the binding mode.On the KPC-2 project we performed an optimization approach using computational methods followed by synthesis of our cooperation partner.

Bergen Und Umgebung, Norwegen

University Of Athens, Department Of Cell Biology And Biophysics

Greek-German Project IKYDA 2013: "Structural and functional Analysis of protein kinase CK2 in the insect Ceratitis capitata and its use as a model molecule in the study of human diseases."

Köln, Institute For Biochemistry

Peptide synthesis and analysis in the working group of Prof. Dr. Neundorf

Master Of Science (M.Sc.), Chemistry

Masterthesis under supervision of Prof. Dr. Karsten Niefind "The extraordinary ATP/GTP-use of Protein Kinase CK2 - a crystallographical.

Bachelor Of Science (B.Sc.), Chemistry

Bachelorthesis under supervision of Prof. Dr. Lars Redecke "Cloning, recombinant gene expression and characterization.