Raul Dominguez-Ramirez, M.S. ,P.E. Email & Phone Number

Houston, Texas, United States

Water storage, conveyance, water and wastewater master plans, H&H Modeling, and design of water and wastewater treatment plants (new, expansions, rehabilitations, or improvements).

Lubbock, Texas Area

My research focuses on the interconnection of natural and synthetic biomaterials to develop novel porous polymeric networks for biomedical and tissue engineering applications. I am also currently involved in characterizing a novel 3D cell culture system for mouse embryonic and induced pluripotent stem cells. Our goal is to determine the effects of the system on the viability, morphology, physiology, pluripotency, and differentiation capabilities of the cells lines. I take advantage of immunobiology, microscopy, biomaterials science, and chemical engineering techniques to determine how we can improve the mechanical and physical characteristics of a polymeric network to execute a particular task; be it drug delivery, vaccinations, or wound healing applications. A particular focus has been given to using chitosan (natural polymer) alongside elastin-like polypeptides (synthetic polymer) to develop temperature sensitive hydrogels with improved mechanical and physical characteristics than previous chitosan gels. My areas of experience include the following:1. Mechanical and physicochemical characterization of biomaterials via rheological techniques, scanning electron microscopy (inorganic and organic samples), transmission electron microscopy (inorganic and organic samples), swelling ratio, porosity, and FTIR.2. Experience in protein purification and characterization using SDS-PAGE, Western Blot, MALDI, UV spectrophotometry, and biological assays.3. Experience in primary myeloma, embryonic, induced pluripotent, fibroblast, and T cell culture.4. Experience in RNA purification and analysis using quantitative real-time PCR.5. Currently undergoing training in multicolor flow cytometry for cell surface and internal protein expression. 6. Currently undergoing training in confocal microscopy.7. Experience in mouse work (lung isolation, spleen isolation, bronchoalveolar fluid isolation, bone marrow isolation, blood drawing).8. Experience with ELISA assays.

Lubbock, Texas Area

Teaching assistant for Engineering Analysis I and various Engineering Seminars.

2700 W W Thorne Dr, Houston, Tx 77073

Tutored in Mathematics, Biology, Chemistry, Biochemistry, and Physics.

Houston, Texas Area

1. Performed research into multiple pathologies including Alzheimer’s Disease, Parkinson’s Disease, Lewy Body Disease, Frontotemporal Dementia, PTSD, ASD, and CTE. 2. Participated and contributed to diverse aspects of clinical research and studies including grant writing and review, data collection and processing, and enrolling patients in clinical studies. 3. Ensured compliance with ethical standards and regulations regarding consent and confidentiality for all clinical study participants.

Springfield, Massachusetts Area

1. Conducted research on protein tyrosine phosphatases (PTPs) in order to develop tools to specifically inhibit and activate genetically engineered PTPs.2. Gained and applied knowledge in various laboratory techniques including gel electrophoresis, Michaelis-Menten assays, in vivo assays, protein purification, cell culture, DNA purification, fluorescence binding assays, and inhibition assays. 3. Defended thesis research for entire biochemistry/biophysics department.

Houston, Texas Area

1. Performed research regarding molecular pathobiology of Aortic Aneurysms & Dissections (AAD) to encourage development of new pharmaceutical strategies preventing AAD formation and progression.2. Observed multiple cardiothoracic surgeries and gained knowledge in the use of laboratory techniques such as western blot, double and triple immunofluorescence, co-immunoprecipitation, and zymography.

Master Of Science - Ms, Chemical Engineering, 3.88

Bachelor Of Arts - Ba, Biochemistry, Biophysics And Molecular Biology, 3.43

Bachelor Of Arts - Ba, Mathematics, 3.43