Ray Ohhashi Email & Phone Number

Newark, California, United States

Novato, California, United States

• Skilled in performance of various analytical methods and molecular biology assays including Immunoassays (e.g., ELISA, BLI), qPCR, ddPCR, UV-Vis Spectroscopy, cell-based methods (e.g. In vitro Cell-Based Potency.
• Executed Method Establishment (Transfer/Qualification/Validation) studies in GxP Laboratory Environment for AAV Gene Therapy Clinical Program in accordance to cGMP and 21CFR guidelines.
• Authored new SOPs and served as SME for Analytical Methods for AAV Gene Therapy Clinical Program.
• Designed and executed Method Optimization and Method Feasibility studies in Lab Notebook studies.
• Authored Method Validation Protocols/Reports in accordance with ICH Q2(R2) guidelines.
• Authored Technical Reports in response to GMP Deviation Investigations.

Santa Barbara, California, United States

• Led QC testing effort during initial product release of Asymmetric-Flow Field Flow Fractionation (AF4) product to market.
• Performed routine QC Testing across different product lines (MALS, RI, DLS, FFF).
• Performed independent analysis of instrument issues and developed solutions in Manufacturing, Service, and Quality Control processes.
• Performed validations of various hardware and software changes.
• Performed method development of QC tests and protocol writing during pilot phase.
• Performed mining of historical QC data and prepared reports on failures and trends.

Santa Barbara, California, United States

Santa Barbara, California Area

• Neuroscience Research Institute, Craig Montell Lab
• Performed In-fusion cloning, E. coli Transformation, Colony Picking, Colony PCR, Plasmid Prep (Mini and Midi scale), and Gel Extraction to support research projects.
• CRISPR plasmid design SnapGene for gene knockout lines in individual project.
• Maintained transgenic lines of Aedes aegypti mosquitoes.
• Refer to Honors and Awards Section for information on research project.

Pleasanton, CA

• Assay development for the detection of infectious agents in the cobas Liat System.
• Examined qPCR Assay performance (e.g., sensitivity, specificity, LOD).
• Tested new reagent formulations and handled clinical samples.