Raymond Hulse Email & Phone Number

As a Senior Scientist with extensive expertise in Molecular Biology and Biochemistry, I have dedicated my career to advancing scientific knowledge through innovative research. I actively focus on developing comprehensive strategies for protein expression and purification, exploring structural biology, and optimizing cell culture techniques.I am passionate about designing and executing cutting-edge experiments that not only drive scientific advancements but also contribute to the development of innovative methodologies. I prioritize operational efficiency by implementing effective laboratory management practices, ensuring smooth logistics and high team performance.Collaboration is key to my approach; I actively foster interdisciplinary partnerships and leverage my expansive professional network to elevate research initiatives. I am eager to drive transformative discoveries that push the boundaries of our understanding in the field of life sciences.Connect with me to learn more about my expertise in the following areas:Research & Analysis | Laboratory Management | Experiment Design Development | Interdisciplinary Collaboration | Presentation & Publication | Research ManagementTechnical Acumen & Lab Techniques:- Molecular Biology and Biochemistry: Cloning (Gibson, Restriction, Golden Gate) | Plasmid Design/Propagation | Western-Blotting | SDS-PAGE | Thermostability and Fluorescent Screening Assays | Activity and Binding Assays | Extension Protein Expression (Cell-Free, Bacterial, Yeast, Insect, Bac-Mam) and Purification Strategies- Proximity Labeling: Generation of Proximity Labeled Proteins | Biochemistry | Analysis of Mass Spectrometry Data- Single Particle Cryo-Electron Microscopy: Negative Staining | Grid & Sample Preparation | Acquisition, Data Processing & Model Fitting/Refinement- Protein Crystallography: Screening, Harvesting | Data Acquisition | Molecular Replacement | Model Building/Fitting/Refinement- Immunohistochemistry & Microscopy: IF | IHC | Live-Cell Calcium Imaging | Confocal & Super-Resolution Modalities (Airyscan, PALM, SIM)- Electrophysiology: Two-Electrode Voltage Clamp | Whole-Cell Patch Clamp | Patch Clamp in Reconstituted Liposomes | Bilayer Recordings & Single Channel Analysis- Spectroscopy: Mass Spectrometry | Continuous Wave & DEER EPR for Mobility | Dipolar Coupling | Distance Parameters | NMR for 1D, 2D, & 3D Experiments for Cross Peak Assignment & Dynamics.- Cell Culture: Primary | Immortalized | Heterologous & Organotypic Cell Culture Systems | Transient & Stable Cell Lines Creation