Raymond Erickson, M.S. Email & Phone Number

St. Paul

Greater Minneapolis-St. Paul Area

Major Projects:• Department of Defense (DoD) Grant pursuing novel therapeutics for recurrent Clostridium difficile (C. difficile) infectiono Primary microbiologist for in vitro sporicidal and inhibition testingo Animal model biologist for C. difficile infection and in vivo therapy• MN-Reach Grant to determine effects of novel C. difficile therapy on human indigenous gut microbiotao Primary microbiologist for in vitro inhibition testing of individual gut microbeso Primary microbiologist for antimicrobial and inhibition testing of whole gut microbeso Primary microbiologist for antimicrobial and inhibition testing of whole gut spore-forming microbesMinor Projects:• Scalp microbiome sequencing of alopecia areata• Repetitve sequence-based PCR DNA fingerprinting of C. difficile genome

Easton, Md

Duties, Accomplishments and Related Skills:• Nucleotide (RNA/DNA) Extraction, Quantification using Spectrophotometry (260/280 ratio), qPCR/RT-qPCR, Gene Expression Analysis (2-∆∆Ct Method)• Aseptic Technique and Molecular Biology Techniques• Precise preparation of stock solutions for aquatic toxicological studies• Coordinate with chemistry liaison and chemists to determine appropriate methodology for preparation of stock and test substances • Preparation and calibration of test substance delivery devices including; saturator column, syringe pumps, FMI pumps etc. • Collection of tissue samples, including: blood, liver, tissue, gonad, etc. • Performing functional solubility trials with test materials• Ordering and stocking laboratory reagents, buffers, supplies, and equipmentGeneral:• Be familiar with pertinent study protocols, GLP's, SOP's and ensure adherence to them in one's own work as well as work received from other individuals• Collect date and record the results on acute, chronic, early-life stage and life-cycle studies• Regularly initiate, maintain, and terminate the in-life phase of assigned tests under the supervision of the Study Director/P.I., and ensure compliance with GLP Standards• Regularly collect data and record the basics such as water chemistry results, e.g. pH, dissolved oxygen, hardness, and salinity

Duluth, Minnesota Area

Duties, Accomplishments and Related Skills:• Nucleotide extraction, quantification using spectrophotometry and nano-drop, and analysis via RT-qPCR• Preparation and evaluation (pH, osmolality, ion concentration) of reagents for trout liver perfusion and slicing • Maintaining bacterial cell cultures• Tissue collection• Keeping experimental records, data collection, and data analysisAdditional Skills• Training in Molecular and Aseptic techniques, including: Ligand Receptor Affinity Analysis, Nucleic Acid Extraction, Isolation, and Quality Evaluation, Gel Electrophoresis, LSC, SDS PAGE, Western Blot assay, in vitro Translation, and RT-qPCR including Primer and Probe Optimization

Master’S Degree, Biotechnology, 3.62 Gpa

Bachelor'S Degree, Cell/Cellular And Molecular Biology