Ray C. Email & Phone Number

Seattle, WA, US

Seattle, Washington, United States

Seattle, Washington, United States

• Generated/maintained various cell line models and developed/optimized cell line based functional assays.
• Assisted with setting up functional assays (sequential kill/serial stims) looking at killing (cytotoxicity by IncuCyte) and cytokine secretion (MSD) for both TIL and PBMC based products.
• Ran a range of flow panels for TIL phenotyping during production and functional assays. (Attune NxT/Cytek Aurora)
• Contributed to molecular biology work when needed (DNA extractions/ddPCR/cloning etc.)

Seattle, Washington

• Contributed to the discovery, validation, and development of cancer immunotherapeutics.
• Responsible for production of recombinant proteins for new antibody therapeutics and reagents with transient transfection and with high producing stable cell lines.
• Assisted in engineering research cell lines for in-vivo animal study and in-vitro cell-based assays.

Seattle, WA

• Assisted in gene-editing projects relating to expression of CD33 for Acute myeloid leukemia (AML) and bcl11a gene therapy for hemoglobinopathies.
• Various molecular/cellular biology responsibilities: cloning/ligations, nucleic acid purification, CRISPR in-vivo and in-vitro gene-editing electroporation/data analysis, ELISA, mouse & NHP blood/tissue processing.
• Tracking of retroviral integration sites in cell populations using Next-Generation Sequencing technology.
• Preparing DNA samples for Illumina platform sequencing through a range of molecular biology techniques: PCR, DNA isolation, qPCR, Tapestation
• Large scale production of retroviruses (foamy virus and lentivirus) for gene therapy/gene editing studies.
• Planned and executed viral vector production and titration ◾ Discussed vector design optimization ideas and concepts

Seattle, WA

• Responsible for overseeing the day-to-day operational needs of the laboratory.
• Assisted in Molecular Biology core: Molecular Cloning, PCR, Gel Electrophoresis
• Helped with Lentivirus Production, Concentration, Titering and Flow Cytometry Analysis

Bainbridge Island, WA

• Executed laboratory activities to support contract and grant research.
• Small scale and large scale fermentation of microbes.
• Collection and maintenance of analytical data.
• Responsible for SDS-PAGE, protein expression testing, Western Blotting, and related molecular biology activities
• Preparation and sterilization of media and stock solutions.
• Clean and maintain all Fermentation/Molecular Biology equipment, supplies, and reagents.

Emeryville, CA

• Processing, pick-up, and delivery of glassware and reagents, and providing quality support to all customers within Norvartis site.
• Followed SLPs and maintained documentation, accurately recording data and data entry to GMP standards
• Used and maintained laboratory equipment daily, and ensured sterility of glassware and reagents by using aseptic techniques or autoclaving procedures
• Worked in a fast paced production environment that demanded a high level of multitasking and flexibility
• Provided accurate work output data to supervisors, assisted with tracking of goals and production.

Waipahu, Hawaii

• Participated in a BSL2 environment for micropropagation of sugar cane and apple bananas.
• Cultivated plant cell tissue cultures for tropical plant research, met production goals and deadlines for shipping.
• Management of samples; analyzed for bacterial/fungal contamination.
• Assisted in lab operations including: media preparation, glassware cleaning, and autoclaving of tools/waste.