Reham Ghali Email & Phone Number

Raleigh-Durham, North Carolina Area

Department of Cellular and Molecular BiologyDesigning and executing experiments to generate recombinant proteins for therapeutics. Collection, evaluation, and interpretation of data generated in order to find new indications. Managing multiple tasks and projects concurrently to meet aggressive deadlines for new targets utilizing recombinant proteins. Presenting data analysis and interpretations to aide in selection of lead candidates. Performing limited dilution cloning to generate monoclonal protein therapies.Utilize QPCR to screen genes of interest and analyze complex pathway specific changes.

Cary, Nc

- Organize and assist with activities for improving physical and cognitive abilities.- Monitor the safety of patients and assist with ambulating.- Provide social interaction to improve memory and cognition.

Project management and progression for target identification and validation studies. Design and execute experiments to elucidate functional roles of various targets of interest. Assess knockdown of messenger RNA using TaqMan Real-Time PCR. Assess protein knockdown/overexpression via Western blotting.. Assay validation and compound mechanism of action. Data collation, analysis and interpretation. Presenting data to project teams and department meetings.Small Interfering RNA (siRNA) library screens. Transfection optimization of primary cells and difficult to transfect cell lines for siRNA screens. Assay validation and optimization for RNAi Screens. Utilize various siRNA libraries such as kinases and phosphatases to find drug potentiators and identify new targets for oncology and metabolic pathways therapeutic areas. Utilize bioinformatic tools for data analysis and statistics. Follow-up with screen hits using various phenotypic assays.

Utilizing RNA interference (RNAi) for knockdown and target/assay validation studies. Assess functional knockdown of various oncology genes utilizing cell-proliferation assays and cell-cycle profiling using DNA content using FACS. Assess downstream effect of knockdown of oncology, diabetes, obesity and inflammation targets. Utilizing viral delivery of short-hairpins (shRNAs) for long term knockdown. Generation of positive and negative controls for using Adenovirus overexpression of shRNAs in-vitro and in-vivo. Assess long-term knockdown for target validation

Generate, amplify, purify and validate recombinant adenoviruses and train others in these activities, Utilize molecular biology (cloning, ligations) techniques for cloning genes of interest into viral vectors (entry and destination vectors). Generation of numerous adenoviruses for internal support and external use by GSK groups and collaborators. Utilize overexpression of genes for target validation alongside small molecules.

Involved in the construction of pre-therapeutic messages for Spliceosome-mediated RNA Trans-splicing gene therapy. Maintained Mammalian cell lines and performed transfections. Assessed effects of mRNA splicing on RNA and reporter genes (B-Gal). Trained new employees in RNA, DNA, and protein isolations. Maintained and ordered lab supplies and equipment.

Abiotic Stress DepartmentGeneration of drought resistant plants, Developed Starch assay for processing plant tissue. Maintained green house experiments under drought conditions. PCR, RT-PCR, RNA/DNA isolation and quantification.

Receptor Biochemistry DepartmentTransfected seven trans-membrane receptors into Xenopus Melanophores. Tested response of cell receptors to various peptide libraries. Learned basic tissue culture and aseptic techniques

Master Of Physician Assistant Practice, Physician Assistant, 3.68

Activities and Societies: Wallace Society

Bachelor Of Science, Biology With Minor In Chemistry

Activities and Societies: Alpha Epsilon DeltaUndergraduate Research Assistant, University of North Carolina – Chapel Hill, Biology.