Scientist Instrumental Bioanalytics

Downstream Processing & AnalyticsHPLC- and LC/MS-based protein analytics, coordination of routine analytics staff, establishing new methodologies

AG Klauschen - Systems PathologyTranslational proteomics with clinical samples, tumor cell enrichment, primary cell culture, proteomic analyses (using Thermo HF-X and QExactive Plus instruments), data analysis (R, MaxQuant), cell cultureFunded partially by DKFZ, DKTK partner site BerlinVisiting Scientist at Proteomics Core Factility / AG Mertins at MDC Berlin-Buch

Dept. Biomolecular Systems of Prof. Peter Seeberger, GPI- and Glycoproteins Group of Dr. PD Daniel Varón SilvaFocus: Protein Expression in bacterial cellsProtein Purification (FPLC)HPLC of Proteins and PeptidesLC-ESI-MS of Proteins, Peptides, complex glycolipidsChemical Biology, Site-Specific Modification of Proteins (Expressed Protein Ligation, Protein Trans-Splicing)Protein Characterization (SDS-PAGE, Western Blot, CD)Molecular Biology

- Fermentation development Fed-Batch-Fermentation (recombinant E. coli, bench scale, parallel fermentation site)- Fermentation in pilot scale (100 L)- Feed automation- Process control- Process analytics (HPLC, enzymatic and photometric assays)- Microbiology (strain maintenance, cryo cultures, selection)

- Whole-cell Biotransformation with different yeast strains- chiral GC, HPLC- Protein purification, protein precipitation

Food analysis (HPLC)Vitamin and Toxin content measurement of different foods (extraction and quantification in HPLC)

Food analysis- Microbiology (enrichment cultures and selection of Salmonella and other bacteria)- Biochemistry (vitamin quantification with Lactobacilli)