Articles Published:1. Sievers RE, Schmiedl U, Wolfe C, Moseley M, Parmley W, BraschR, Lipton M. A modelof acute regional myocardial ischemia and reperfusion in the rat. Mag Res in Med. 10:172-181, 1989.2. Heiss C, Sievers RE, Amabile N, Momma T, Chen Q, Natarajan S, Yeghiazarians Y, Springer M: In vivo measurement of flow-mediated vasodilatation in living rats using high resolution ultrasound. Am J Physiol Heart Circ Physiol 294: H1086-1093, 2008. 3. Mihardja SS, Sievers RE, Lee RJ: The effect of polypyrrole on arteriogenesis in an acute rat infarct model. Biomaterials 29: 4205-4210, 2008.4. Mihardja S, Gao D, Sievers RE, Fang Q, Feng J, Wang J, Dae M, Lee R: Targeted In Vivo extracellular matrix formation promotes neovascularization in a rodent model of myocardial infarction. PLoS ONE 5(4): e10384, 20105. Zhang Y, Sievers RE, Prasad M, Mirsky R, Shih H, Wong M, Angeli F, Ye J, Takagawa J, Koskenvuo J, Springer M, Grossman W, Boyle A, Yeghiazarians Y. Timing of Bone Marrow Cell Therapy is More Important Than Repeated Injections after Myocardial Infarction” Cardiovascular Pathology 2011 Jul-Aug;20(4):204-12.6. Gaur M, Ritner C, Sievers R, Pedersen A, Prasad M, Bernstein HS, Yeghiazarians Y. Timed inhibition of p38MAPK directs accelerated differentiation of human embryonic stem cells into cardiomyocytes. Cytotherapy. 2010 Oct;12(6):807-17.7. Huang N, Sievers RE, Park J, Fang Q, Li S, Lee R: A rodent model of myocardial infarction for testing the efficacy of cells and polymers for myocardial reconstruction. Nature Protocols; Vol.1 No.3, 1596-1609, 2006.8. Qiumei Chen, Sievers RE, Varga M, Kharait S, Haddad D, Patton A, Springer M: Pharmacological inhibition of S-nitrosoglutathione reductase improves endothelial vasodilatory function in rats in vivo. J Appl Physiol 114:752-760, 2013.

1. Lum L, Fok H, Sievers RE, Abedi M, Quesenberry P, Lee R: Targeting of Lin-Sca+hematopoetic stem cells with bispecific antibodies to injured myocardium. Blood cell, Molecules, and Disease. 32:82-87, 2004.2 Christman K, Vardanian A, Sievers RE, J fang Q, Fok H, Lee R: Injectable fibrin scaffold improves cell transplant survival, reduces infarct expansion, and induces neovasculature formation in ischemic myocardium. JACC 44:654-660, 2004.3. Christman K, Fang Q, Yee M, Sievers RE, Lee R: Enchanced neovasculature formation in ischemic myocardium following delivery of pleiotrophin in a biopolymer. Biomaterials 26(2005) 1139-1144.4 Altman R, Sievers RE, Lee R: Exploring heart lymphatics in locals drug delivery. Lymphatic Research And Biology January, Vol. 1 (2003), No.1, 47-54.5 Christman K, Fang Q, Kim A, Sievers RE, Fok H , Lee R: Pleiotrophin induces formation of functional neovasculure in vivo. Biochemical and Biophysical Research Comm 332 (2005) 1146-1152. 6 Springer M, Sievers RE, Viswanathan M, Yee M, Foster E, Grossman W, Yeghiazarians Y: Closed-chest cell injections into mouse myocardium guided by high-resolution echocardiography. Am J Physiol Heart Circ Physiol 289:H1307-1314, 2005.7. Chen Q, Sievers RE, Varga M, Kharait S, Haddad D, Blonder J, Dube G, Rosenthal G, Springer M: Pharmacological Inhibition of S-Nitrosoglutathione Reductase Improves Endothelial Vasodilatory Function in Rats in vivo. J Applied Physiology, 2013.

Research Techniques:Developed the ischemia-reflow model in the rat which characterized the snare loop occlusion of the left coronary artery. By using the rat, this technique resulted in lowered costs and higher throughput with pharmaceutical interventions. Pioneered a technique for in vivo gene therapy transfer directly into the AV node. We have extended this knowledge to include the feasibility for a therapeutic role for cell transplantation and gene transfer in myocardial repair.Developed a chronic model in the rat to assess the effects of pharmaceuticals on left ventricular remodeling.Developed a closed-chest cell injection technique into mouse myocardium guided by high-resolution echocardiography. The use of this technique will allow more precise cellular implantation into the mouse/rat myocardium by accurately guiding injections to desired locations, confirming successful implantation of stem cells, in a clinically relevant time frame.Developed the first integrative physiologic model to reproducibly study Flow-mediated dilation in living rats. This approach demonstrates that the physiology of rodent vascular reactivity is concordant with that of humans, validating its use for the study of therapeutic modulators of vascular function.