Richard Olsen Email and Phone Number

Continue lab responsibilities of Biochemist 3aLead and support group of six individual contributorsSchedule group meetings, 1:1 meetingsProvide New Employee, Mid-Year, and Annual Reviews, discuss strengths and opportunities with each group memberCoordinate with each client area to discuss needs, current performance, and opportunities

Continuing duties of Biochemist 2bIncreasing responsibilities with microbial culturesIncreased cross training in large scale (250-500L) mammalian culturesMember of Safety Team. Responsible for conducting new employee safety orientation, covering safety topics, lab walkthroughs and coordinating with the client to document training completion.

Continuing duties of Biochemist 2aInitiating and conducting investigationsRunning, maintaining, and troubleshooting NovaFLEX 2, MAST autosampler and Bio HT analyzerRunning, harvesting, and purifying E. coli cultures for insulin studies.

Working with mammalian CHO cells in drug discovery by performing shake flask studies and executing 5L, 36L, and 250L bioreactor studiesBuilding, running and harvesting bioreactors in various settings, coordinating with other scientists using Levtec bags, TFF filtration, and ProA column.Documenting protocols and resultsSample submission and LIMS data entryReviewing notebooks and reports

a. Working with the ATD RTA (Rapid Testing Assay) team challenged with developing enabling technologies for screening zinc finger nuclease (ZFN) designs and ExZACT component testing in DAS crops (maize, soybean, and canola). b. Presented findings in team meetings to assist in the decision making of the relative performance of different culture lines and new methods.c. Trained six interns (2009-2014), including safety policies and procedures, the maintenance of suspension cultures, single cell isolation/ transfection, and flow cytometry characterization of cells.d. Responsible for high throughput bulking, extraction and purification of 100s of plasmids using both kits and automation. Presented status updates to the groups to facilitate smooth process flow.

Feeding and care for species in the Animal Encounters area.Teaching and interaction with zoo visitors.Required a high level of initiative and autonomy in carrying out responsibilities.

• Research on the location and movements of grizzly bears in northwest Montana.• Hiked through the backcountry and set up hair snag sites.• Required problem-solving, teamwork, and intense dedication.