Rishabh Shetty Email & Phone Number

Bengaluru, Ka, In

Pleasanton, California, United States

San Diego, California, United States

• Responsible for immunoassay design, reagent development, systems integration, proof-of-concept, and process improvements (passivation, conjugation chemistry, efficiency, and backgrounds) to benchmark performance of solution-phase technologies in buffer and plasma/serum using PCR-based techniques.• Compared in-house protein quantification immunoassay performance in various formats with aptamer-based and antibody-based affinity binders against standard ELISAs and proximity-based assays. • Helped implement DNA nanotechnology techniques to detect leaks on DNA microarrays for quality control (Q2 2024 release).

San Diego, California, United States

San Diego, California, United States

• Employee #1 at DNA Nanotechnology-based startup (acquired in 2022) focused on developing a platform to enable large scale, highly multiplexed proteomic quantification.• Part of the core scientific founding, hiring and onboarding, systems integration, planning, and intellectual property (co-inventor on multiple systems & methods invention disclosures) teams.• Led and co-led the development (2 direct reports and team of 5) and scale-up of surface and solution-based molecular quantification assays using single-molecule placement & protein-to-DNA conversion techniques. • Single-molecule placement achieved was ~90% and demonstrated a fully scalable method on semiconductor chips for creating super-Poisson DNA nanoarrays for biomedical applications.

San Diego, California, United States

Scaling up semiconductor manufacturing of chip-based DNA biosensors for proteomic applications.

Pasadena

• Developed and validated a design for thermostable DNA origami to improve sample preparation in next-generation sequencing by enabling monoclonal cluster generation in solution or solid surfaces.• The proposed method could potentially solve the problem of low-efficiency, polyclonal sequencing technologies that exist on the market today, while extending the field-of-use of DNA origami techniques.• Mentored a PhD candidate on methods (published in Langmuir) of rational fabrication of polycrystalline opals using self-assembly for applications in optical coatings, thermofluidic devices, and biomolecular templating.

Tempe, Arizona

Center for Molecular Design and Biomimetics (MDB), The BIONICS lab (http://hariadilab.strikingly.com/)• A modular DNA origami nanoarray platform for low-cost diagnostics and high-throughput single-molecule biophysics1. Developed a ~$1, cleanroom-and lithography-free chip-based DNA origami nanoarray device using a process of nanosphere self-assembly. 2. Conducted device characterization using Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), bright field- and Total Internal Reflection Fluorescence (TIRF)- microscopy.3. Experimental characterization and optimization were performed to quantify yield of single molecule binding, single-stranded DNA conjugation efficiency, and in situ isothermal amplification reactions through cyclical strand displacement.4. Performed high-throughput single-molecule experiments such as diffraction-limited Holliday Junction Förster Resonance Energy Transfer (FRET), and super-resolution DNA-Points Accumulation for Imaging in Nanoscale Topography (PAINT) to highlight platform versatility.5. Demonstrated non-Poisson-limited single molecule binding efficiency with >2 orders of magnitude increase in data density versus traditional techniques, and >100x cost reduction versus currently used top-down lithography-based fabrication techniques.Mentoring:1. Mentored ASU Barret’s Honors College undergraduate students and Flinn Scholar award recipients (six in total), with their laboratory activities and research projects. 2. Managed laboratory equipment and was involved in equipment maintenance, raw material procurement and stocking, documentation of experimental procedures and general lab maintenance duties. 3. Demonstrated the capabilities of a benchtop super-resolution microscope for single-molecule biophysics and cellular cytoskeleton imaging to visiting high school students and freshmen.

Tempe, Arizona

• Optical computed tomography for spatially isotropic four-dimensional imaging of live single cells1. Co-developed an instrument setup to facilitate isotropic live cell computed tomography for diagnostic and prognostic purposes. Optimized the cell rotation platform – a dielectrophoretic “electrocage” chip-based setup – based on rotation speed, stability, and cell stress for live myelogenous leukemia cells through tunable parameters such as electric field amplitude and frequency at a stable rotation rate of 1.5 to 2 rpm.2. The quantitative data obtained using this system are amenable for multiparameter characterization of cellular phenotypes and kinetics in the clinical setting, and is the first reported technique enabling live, 4D immune system cell imaging at the diffraction limit.• Microfabrication methods to generate out-of-plane microvortices for single cell rotation and 3D imaging1. Investigated three different microfabrication technologies for manufacturing out-of-plane, undercut trapezoidal structures for generating microscale vortices applicable to 3D single cell imaging with high spatial resolution.2. Characterized the suitability of each process based on reproducibility, cost efficiency, feature geometry, and time consumption, and fabrication flexibility. 3. Optical tweezers were utilized for stabilizing cell rotation and the rate of rotation was assessed as a function of differing linear flow rates, optical trapping intensities, and feature geometries.• Multiple sensor arrays for single cell metabolic analysis1. Aided in the fabrication and characterization optimization of multiple “micro-pocket” lid arrays with application in multiparametric live single cell metabolic analysis. 2. Two step photolithography and wet chemical etching were utilized to accommodate multiple sensors such as oxygen, pH, and other extra-cellular sensors at dual depths in a hermetically sealed glass lid for reduced cytotoxicity.

Tempe, Arizona

1. Lectured on three topics for a graduate level Biomedical Microdevices course, and graded homework assignments for the course.2. Helped design and grade the mid-term, final exams, and assisted in the evaluation of final class projects.3. Held regular office hours for students requiring assistance with course materials and final projects.

Tempe

Delineation of somatosensory finger areas using vibrotactile stimulation1. Aided in the design of a vibrotactile stimulation device for delineation of somatosensory finger areas as an alternative to direct electrical stimulation of the brain.2. The custom finger pads were designed on AutoCAD and tested on several patients to test its ergonomics.3. Electrocorticographic monitoring was performed while patients’ fingers were independently stimulated and the elicited activity was recorded for downstream statistical analysis.4. The study was designed to provide a faster and more objective map to better anticipate outcomes of surgical resection.

Tempe

• Assisted instructors in teaching and grading five ASU undergraduate courses: MAT 117, 142- College Algebra, MAT 194 - Enhanced Freshman Mathematics, MAT 210- Differential and integral calculus of elementary functions and applications, and STP 226 - Elements of Statistics.

Mumbai Area, India

Built a cost-effective,portable and efficient Automated Cardiopulmonary resuscitation Device (A-CPR). Used Aluminium and Stainless steel as the basic materials which were then machined to form the basic components of the system.The system consisted of a rotating disc,an Applicator arm,a base plate,two adjustable stands to make the instrument portable and a motor to drive the applicator arm.The timing of the compression to be delivered,according to WHO guidelines was controlled using an IC 555 multivibrator.

Doctor Of Philosophy - Phd, Bioengineering And Biomedical Engineering, 4.0

Performed research in the diverse areas of single molecule biophysics, low-cost diagnostics, DNA nanotechnology, diffraction-limited.

Master Of Science (M.S.), Bioengineering And Biomedical Engineering, 4.0

Biomedical Engineering with research in the field of microfabrication, microfluidics, and single cell imaging/manipulation (fluorescence.

Bachelor Of Engineering, Biomedical Engineering

Activities and Societies: Project entitled 'Automated Cardiopulmonay Resuscitation Device (A-CPR) was presented at the final round.