Over 30 Years in Field of Flow Cytometry and Laboratory Science: Clinical (CAP/CLIA), Pre-clinical (GLP) Toxicology, Discovery Biology/Pharmaceutical Research, CRO (Contract Research Organization) Technical Expertise:▪ Flow cytometry instrumentation: Beckman CytoFLEX LX (12C - 6 laser), CytoFLEX S (13C-4 laser), CytoFLEX (13C-3 laser), Coulter Gallios (10 color), BD FACSCanto II (8 color), Coulter FC500 (5 color), Coulter EPICS XL-MCL (4 color), BD FACScan (3 color), Coulter EPICS Elite cell sorter (3 color), Coulter EPICS Profile I (2 color).▪ Flow cytometry cell sorting: CytoFLEX SRT benchtop sorter (Beckman Coulter), FACSAria cell sorter (BD Biosciences), Miltenyi AutoMACS.Automation: Beckman Coulter Biomek i7, Curiox laminar flow cell washer.▪ Flow cytometry off-line analysis software: Cytobank (Beckman Coulter), WinList (Verity Software House), Kaluza (Beckman Coulter), FlowJo (Tree Star), ModFit LT (Verity Software House).▪ Flow cytometry applications: immune cell phenotyping / immune cell function, Th1/Th2/T-reg, Naïve/memory/activated T-cell and B-cells, Eosinophils, Activation/Adhesion (PDL1, CD69, CD25). Intracellular cytokine, Receptor occupancy, Hematological malignancies (AML, CLL, MM, ALL), Signal transduction/Cell signaling (Phospho-epitopes), DNA Cell Cycle/Proliferation (Ki67, CFSE, BrdU, EdU, PI, DRAQ5), Apoptosis (Caspase 3, Annexin V), Rare event (Progenitor cells, tumor cells, stem cells, dendritic cells), NK cell function, Platelet activation (CD62P, PAC-1, fibrinogen), neutrophil (CD11b), monocyte M1/M2 macrophage, microparticle detection.▪ Protein biochemistry: ELISA, Luminex.▪ General Laboratory experience: Tissue culture (adherent and suspension cells), Tissue dissociation, Human and animal (non-human primate, dog, rat, mouse, pig) blood, tissues, and body fluid experience, Isolation of blood leukocytes, Hematology, Coagulation, Clinical chemistry auto-analyzer experience.
Listed skills include Microsoft Office, Management, Microsoft Excel, Microsoft Word, and 24 others.