Riya Rakshit Email & Phone Number

Hyderabad, Telangana, India

India

Key Responsibilities:➤Method Development and Qualification: Developing and qualifying methods using Mass Spectrometry (Orbitrap) for various applications, such as intact mass analysis, reduced mass analysis, subunit mass analysis, top-down sequencing, peptide mapping, and disulphide bridge mapping. Optimizing and validating these methods to ensure accurate and reliable results.➤Impurity Characterization: Characterizing impurities in monoclonal antibodies, fusion proteins, and peptides using Mass Spectrometry techniques.➤Peak Identification and LC Method Development: Supporting the LC team by identifying and interpreting peaks in chromatographic data. Assisting in the development and optimization of LC methods, ensuring efficient separation and detection of analytes.➤Software Utilization: Utilizing software platforms such as Biopharma Finder, Proteome Discoverer, Mascot, Xcalibur, and Chromeleon for data processing, analysis, and interpretation.➤Molecule Analysis: Handling and analyzing various biomolecules, including monoclonal antibodies, fusion proteins, and peptides, using Mass Spectrometry techniques.➤Multiple Attribute Method Development: Developing multiple attribute methods to assess various post-translational modifications (PTMs) and glycans in biomolecules. Optimizing methods to ensure comprehensive evaluation of PTMs and glycans, contributing to product quality control.➤Bio-Similarity Studies and Stability Studies: Contributing to bio-similarity studies to assess the comparability of biopharmaceutical products. Participate in stability studies to evaluate the long-term stability and degradation patterns of biomolecules. ➤Equipment Installation and Qualification: Supporting the installation and qualification of Mass Spectrometry equipment. Preparing necessary documents and reports, ensuring adherence to standard operating procedures and regulatory requirements.

Ahmedabad, Gujarat, India

Ahmedabad, Gujarat, India

Key Responsibilities:➤Method Development and Qualification: Developing and qualifying methods for protein characterization using a variety of techniques, including Analytical Ultra Centrifugation (AUC), Far and Near UV Circular Dichroism (CD), Differential Scanning Calorimetry (DSC), Dynamic Light Scattering (DLS), Fluorescence Spectroscopy, UV spectroscopy, Free Cysteine estimation, SEC MALS (Multi-Angle Light Scattering), and various chromatography techniques (CEX, SEC, RP) using HPLC and UHPLC. ➤Molecule Handling: Handling a range of molecules, such as Adalimumab, Ranibizumab, Bevacizumab, GCSF, Peg GCSF, Icatibant, and Virus particles. Performing characterization and analyzing on these molecules using the developed methods and techniques.➤Planning and Execution of Studies: Playing a key role in planning and executing studies, including stability studies, hold time studies, and force degradation studies. Contributing to experimental design, data analysis, and interpretation to assess product stability, degradation pathways, and potential challenges.➤Regulatory Support: Supporting the HOS (Higher Order Structure) and Chromatographic teams in regulatory submissions, bio-similarity assessments, and addressing regulatory queries.➤In-house Method Development and Cost-Cutting: Contributing to cost-cutting efforts by developing in-house methods that were previously outsourced. Optimizing and establishing analytical techniques, such as using the Beckman Coulter OPTIMA AUC, to bring efficiency and cost-effectiveness to the analytical development process.➤QMS Elements and Recognition: Handling Quality Management System (QMS) elements related to analytical development and demonstrating compliance with relevant guidelines and regulations.

Hyderabad, Telangana, India

Key Responsibilities:➤Clone Development and Protein Expression: Participating in the development of clones for the vaccine candidate. Conducting protein expression experiments in shake flasks to produce the target protein. Optimizing expression conditions to achieve high protein yield.➤Purification and Characterization: Utilizing NGC Chromatography for the purification of the vaccine candidate protein. Implementing purification protocols to isolate and purify the protein of interest. Performing characterization studies using techniques such as Circular Dichroism (CD), Differential Scanning Calorimetry (DSC), and Dynamic Light Scattering (DLS) to evaluate its structural properties and stability.➤In-vivo Mice Studies: Planning and conducting mice studies to assess the efficacy of the vaccine candidate. Optimizing the dosage of the vaccine for maximum immunogenicity. Analyzing immunogenicity responses in mice to evaluate the potential effectiveness of the vaccine in combating the deadly carcinoma.➤Downstream Processing Protocol Standardization and Scale-up: Standardizing the downstream processing protocol for the vaccine candidate. Developing and optimizing the purification process to ensure reproducibility and efficiency. Successfully scale up the process to a 10 L batch, enabling larger-scale production of the vaccine candidate.➤Expression and Purification of Yeast Recombinant Antigen: Handling the expression and purification of a yeast recombinant antigen. Standardizing yeast growth kinetics and transformation techniques, such as electroporation, to achieve efficient protein expression. Purifying the recombinant antigen to obtain a high-quality and pure protein product.➤Career Advancement: Recognized for success with the vaccine development process hence, promoted from a Trainee to a Research Associate. This promotion acknowledges my contributions and achievements in successfully advancing the vaccine development project.

Hyderabad Area, India

Hyderabad, Telangana, India

Key Responsibilities:➤Expression and Purification: Successfully expressed and purified CheY proteins using affinity chromatography techniques, specifically with AKTA prime system. This involved optimizing expression conditions, protein extraction, and purification methods to obtain high-quality CheY protein samples.➤Analysis of Protein Structure: Employed various analytical techniques to analyze the structure of CheY proteins in the presence of a crowding agent. These techniques include:➤Circular Dichroism (CD): Used to assess the secondary structure and conformational changes in CheY proteins. CD spectroscopy provides information on protein folding, stability, and interactions.➤Differential Light Scattering (DLS): Utilized to measure the size distribution and aggregation state of CheY proteins. DLS can indicate protein-protein interactions and the effects of crowding agents on protein stability.➤Dynamic Scanning Calorimetry (DSC): Employed to investigate the thermal stability and folding/unfolding behavior of CheY proteins. DSC measures the heat capacity changes associated with protein conformational transitions.➤Fluorescence and UV Spectroscopy: Employed fluorescence spectroscopy to probe the tertiary structure and tryptophan environment of CheY proteins. UV spectroscopy was used for protein quantification and determining the concentration of CheY samples.➤Protein Quantitative/Qualitative Assay: Utilized assays to quantitatively determine the concentration of CheY proteins. This helps in accurate sample preparation and data analysis.➤Analysis of Crowding Agent Effects: Investigated the influence of the crowding agent on the structure and stability of CheY proteins. By comparing the results obtained in the presence and absence of the crowding agent, you have assessed its impact on protein conformation, folding, aggregation, and overall stability.

• Teaching Assistant in Department of Mathematics• Teaching Assistant in Work Integrated Learning Programs• Teaching Assistant in Department of Economics and Finance• Teaching Assistant in Department of Biological Sciences

Industrial training on production of Quin-Iodochloro IP and colloidal Iron

Standardizing amplified restriction fragment length polymorphism of sesame in agarose gel• Construction of SSR enriched genomic library by different methods.• Identification of valid SSR, ISSR and RAPD markers in sesame.• Morphological and molecular characterization of a core collection of sesame.

Kolkata Area, India

Cytotoxic effect of Madhuca indica extracts on HCT-116 colon carcinoma cell line.Cell revival and maintenance, Cytotoxicity assay, Viability assay, Immunofluorescence staining, Hematoxylin Staining for Apoptotic Nuclei dectection, Western Blot

Master Of Engineering - Me, Biotechnology

Genetic polymorphism studies of RON2 protein in Indian field isolates of malarial parasite Plasmodium falciparum; structure prediction.

Bachelor Of Technology - Btech, Biotechnology

Efficacy of in-situ formulated silver nanoparticle as an antibacterial agent on the leaf extract of Azadirachta indica • Ranked 3rd.