● Support upstream and purification group within Viral Vaccine Process Development and Commercialization.● Applied GMP/GLP in areas as appropriate, used Aseptic techniques for all cell work.● Designed and executed cell-based assays for efficacy, potency and GMP lot release.● Regularly utilized human cell lines for cell culture processes using live virus infection.● Generated and maintained human cell lines for studies.● Performed Cell Culture and Virus purification assays.● Author technical documents detailing process to support filing.● Performed Flow Cytometry (FACS) staining and analysis for cell culture infection studies.● Generated, analyzed, and interpreted data generated for release validation.

● Performed Flow Cytometry (FACS) staining and analysis for in vitro and in vivo experiments.● Generated, maintained, and cultured mouse and human frozen cell lines.● Performed skin graft surgery on mice, as well as bone marrow and spleen cell culture expansion.● Utilized immunohistochemistry techniques for tissue culture and section creation for fluorescent microscope imaging.● Performed mouse colony management, genotyping, and maintained laboratory animal inventory● Performed cell-based assays including proliferation, differentiation, labeling, and cell death.● Performed ELISA, DNA/RNA isolation, RT-PCR, qPCR, and Cell Culture Assays regularly.● Generated, analyzed, and interpreted data generated for scientific journal submission.● NIH Grant Recipient

● Performed Isoelectric Focusing for protein separation for Quality Control testing.● Performed tests for Absorbance Density of product at 280nm.● Performed testing of product, media, and water samples using membrane filtration and direct plating techniques.● Performed Gel Clot and Limulus Amebocyte Lysate Assays to test samples for Endotoxin.● Conducted environmental monitoring of product manufacturing areas including clean rooms, including air and water surface sampling of manufacturing and laboratory areas.● Managed incoming and outgoing samples using gLIMS for various biological and chemical testing. ● Used Aseptic Techniques for all product and environmental testing.