Robert Sprung Email & Phone Number

Over two decades experience in the biochemical and proteomic characterization of cell lines and human tissue.Custom formulation of insect and mammalian cell culture media for metabolic labeling and growth characterization experiments.Design and execution of Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC) experiments. Kinetic analysis of enzymatic activity using radiolabeled and chromogenic substrates. Photometric determination of ATP release from red blood cells. Milligram-scale gel electrophoresis for fractionation of protein from cell lysates. Milligram-scale HPLC and isoelectric focusing of peptides from formalin-fixed paraffin-embedded and frozen tissue digests for biomarker discovery. Purification of mitochondria from cultured mammalian cells.MALDI, LC-MS/MS and targeted MS analysis of small molecules, cell culture digests, formalin-fixed paraffin-embedded tissue and frozen tissue lysates. Manual validation, analysis and organization of proteomics data. Design and successful execution of independent and investigator-originated research. Editing and writing of scientific papers and grant applications.Laboratory and personnel management and budgeting.

Listed skills include Protein Chemistry, Proteomics, Mass Spectrometry, Analytical Chemistry, and 9 others.