Roberta Pointet Email & Phone Number

-Prescreened clinic lists to identify eligible patients using protocol specific inclusion and exclusion criteria for participation in the Quebec Pancreas Cancer Study (QPCS) and its affiliate studies, documenting each potential participant's eligibility or exclusion -Approached and enrolled eligible patients into the QPCS and its affiliate studies.-Navigated patients through genetic testing and study participation requirements by scheduling and coordinating clinical research appointments, genetic testing and biospecimen collections as required by the protocol -Completed participant enrolment by 1) obtaining authorization for medical records as well as access to archived biospecimens and 2) sending requests for medical records and/or archival biospecimen acquisitions-Collected and performed data entry and management of online study databases-Prepared other study materials as requested by the PI. These study materials include, but are not limited to informed consent documents, case report forms (CRFs), screening and enrollment logs, and SOPs-Establish and organize study files, including but not limited to, regulatory binders, study specific source documentation and other materials-Assisted with returning of genetic testing results to patients and treating physicians

Ottawa, Canada Area

-Assumed the role of the safety representative and co-manager for the lab, responsible for handling orders and various other duties including communicating with sales representatives to learn about new reagents and leverage discounts for bulk purchases, working with other staff to ensure equipment is kept in working order, and record keeping -Maintained multiple mouse colonies of different genotypes and ensured that the lines were true bred by genotyping mouse pups -Worked with researchers to support cancer and human embryonic stem cell and induced pluripotent stem cell research programs-Collected, processed and cryopreserved umbilical cord blood samples, then performed hematopoietic cell lineage depletion of the umbilical cord blood to retrieve stem cells-Transfected HEK 293T cells and transduced lineage depleted cord blood stem cells-Extracted RNA from transduced cells then converted to cDNA to perform qPCR

Ottawa

-Performed site-directed mutagenesis on functional translation proteins to determine their structure, binding domains and function by transfecting mutations into cell lines and conducting immuno-precipitations on cell lysates then visualizing by Western blotting

Ottawa, Canada Area

-Infected and measured infection rate of multiple cancer cell lines including mouse and human gliomas, neuroblastomas and lymphomas with oncolytic viruses Herpes Simplex Virus 1 and Vesicular Stomatitis Virus -Performed Western blots to quantify infection of cell lines -Induced combination therapy with a drug to enhance the oncolysis of the cancer cell lines for preliminary data

-Prepared albumin-based nanoparticles using bovine serum albumin to differentiate between critical quality attributes in the presence and absence of fatty acids -Performed BCA Assays to determine protein concentration by spectrophotometry -Characterized nanoparticle size and stability using NanoSight NS300 instrumentation -Analyzed doxorubicin loading and release of nanoparticles at different concentrations

-Extracted and purified plasmid DNA-Prepared high titer viruses-Transfected HEK 293T cells and transduced lineage depleted cord blood stem cells-Collected, processed and cryopreserved umbilical cord blood samples, then performed hematopoietic cell lineage depletion of the umbilical cord blood stem cells-Collected, processed and cryopreserved leukemic stem cells from peripheral blood and bone marrow aspirate samples-Assisted in creating a bone marrow library-Plated, passaged and cultured HEK 293T cells and TEX cells