Roberta Bosotti, Phd Email & Phone Number

Italy

The Bioinformatics Italian Society (B.IT.S) is a non profit association with the aim of promoting meeting and discussion among researchers interested in different aspects of the bioinformatics, a multidisciplinary science that study biological problems at molecular and cellular level using computational and informatic models and methods.

Pavia, Lombardia, Italia

Math commission coordinator. Member of Director's staff.

Nerviano (MI)

Responsible of genomics and bioinformatics activities in support to NMS projects, coordinating the activities of a team of five people: two dedicated to Genomics, with a focus on biomarkers identification and new target characterization, and three to Next Generation Sequencing (NGS) and data analysis.Group capabilities include: Sanger sequencing and.

Nerviano (MI)

Genomics Group Leader, in charge of genomics and bioinformatics support to project activities.Responsibilities include the use of bioinformatics approaches with the aim of identifying predictive and/or prognostic biomarkers and the supervision of DNA sequencing and synthesis activities, gene expression analysis by RT-qPCR and microarrays. Analysis of Next.

In charge of bioinformatics and statistics support to project activities, from early phase to the clinics. Statistical and functional analysis of gene expression data (microarrays, RT-qPCR) in preclinical experiments in order to characterize drug mechanism of action, facilitate the identification of potential side effects and identify biomarkers, then.

Provision of optimal support for company research projects, through in-depth bioinformatics analysis on genes and proteins, and the implementation of databases for biological knowledge storage.Actively and successfully involved in data mining of Celera database, for the identification of new genes, potentially interesting as new oncological targets. One of.

Stockholm, Sweden

Comparative genomics analysis at CGR (Center for Genomics Research), Karolinska Institutet, Stockholm (Sweden), under the supervision of prof. Erik Sonnhammer.Sequence alignment, phylogenetic analysis, similarity searches (BLAST, HMMER) and protein domain characterization were applied for the identification of a novel homology domain in the N-terminal.

In charge of cloning and expressing cyclin dependent kinase family members (CDKs) as recombinant proteins either in bacterial (E.coli) or mammalian system (COS-1 cells).Key skills: Molecular biology techniques (gel electrophoresis, enzymatic analysis, PCR, DNA cloning, gel elettroforesi, SDS-PAGE,, Western blot, protein affinity purification) and cell.

Yeast Two-hybrid screening technology was applied to identify new binding partners of the ZAP-70 tyrosine kinase.Key skills: DNA manipulations, expression of recombinant protein in E. coli, yeast and mammalian cells, Two Hybrid System, Western blot, protein affinity purification, In vitro kinase assay

A mutational analysis on recombinant ZAP-70, a protein tyrosine kinase that mediates T-cell antigen receptor (TCR) signalling, was carried out with the aim of better characterize its function in the signal transduction pathway.The mutants were cloned and the obtained plasmids were transfected either in E.coli or mammalian COS-1 cells. The recombinant.

Education record

Education record

Education record