Sep2022 to current. Biovision Batch Production Group.Measure liquids and weigh chemicals then add to batch. Make pH adjustments, bring to final volume. Sterile filter into containers.Obtain QC samples of batches post-filter using sterile technique.Aliquot small volumes of formulations into vials for lyophilization.Cap lyophilized vials. Label vials.Jan2022 to Sep2022Manufacturing and in-process and final QC testing of Protein Analysis kits.Analysis of QC results for complex multi-analyte plate assay. Pipetting small volumes of antibody conjugated particles.Aliquoting and labeling of kit components.Operate plate-based automated equipment such as Tecan and Hamilton.

Perform pH and sodium chloride concentration testing on in-process and post-fill formulations.Conduct volume checks of filled syringes of hyaluronic acid medications for final release.Verify all pipettes for monthly calibration. Verify all analytical balances for weekly calibrationPerform raw material testing such as manual titrations of hydrogen peroxide and sodium hypochloride.Maintain lab notebook with testing results.Cleaned lab glassware.

Prepare RNA nanoparticle based formulations. Perform stability studies on Formulations.Test and analyze RNA formulations using Ribogreen plate-based fluorescence assay.Perform particle size analysis, PDI and zeta potential on formulations.Prepare buffers and solutions, test and adjust pH. Weigh solids and liquids in microgram range, routinely pipette in microliter range.

Follow detailed work instructions and SOP's.Work in clean room environment under cGMP.Operate speedmixer, scale, ovens.Manufacture microfluidic devices for DNA sequencing.Prepare reagent batches of silicone and epoxy.Set up fixtures, cast silicone and epoxy molds.

Prepared large and small batches of glucose control solutions, calibrators and standards by cGMP in a FDA regulated lab.This included: weigh chemicals, add to batch, bring to final weight, make pH adjustments.Performed in-process QC testing of pH, conductivity, viscosity, glucose and electrolytes.Use spectrophotometer for absorbance testing.

Prepared buffers and standard calibrating solutions, including serial dilutions. Used pH and conductivity meters. Used pipettes and balances.Performed optical alignment, calibration and troubleshooting of HPLC detectors. Aligned optics including gratings, lenses and mirrors.Troubleshoot and maintain reactor sample systems. Maintain analytical devices such as nutrient monitors (Nova, YSI) and HPLC systems (Agilent, Shimadzu).

Prepared DNA for sequencing, quantitated by agarose gel electrophoresis. Performed chemical reactions for DNA amplification using PCR. Manually load samples into ABI DNA sequencer using multichannel syringe. Calibrated and repaired lab equipment.