Rob Sturm Email & Phone Number

Kalamazoo, Mi, Us

Parsippany, Nj, Us

- Leads technology agnostic Translational Biomarkers Team within the Discovery Sciences and Technologies group.- Expertise in quantitative determination of biologics and biomarkers by LCMS.

Durham, North Carolina, Us

- Method development scientist experienced in collaborating with internal and external Sponsors to develop selective, high-throughput, quantitative LCMS assays for peptide/protein therapeutics and biomarkers in support of preclinical and clinical programs.- Developed assays range in complexity from simple protein crash/conventional-flow chromatography to complex offline Immunoaffinity (IA) purification coupled to multi-dimensional (2D/3D) online immunocapture, trap and elute nano-flow chromatography.- Provides de novo method development support to Sponsors seeking quantification of a target protein therapeutic/biomarker including determination of optimal digestion strategy for production of a signature peptide for use in bioanalysis.- Supports business development and marketing initiatives through onsite/offsite scientific and capabilities presentations.- Collaborates with instrument and reagent vendors to evaluate new technologies for use in a regulated high-throughput laboratory.- Authors SOPs and work instructions pertaining to implementation of high resolution accurate mass spectrometry (HRMS) instrumentation and software for regulated bioanalysis.- Leads instrument validation activities for equipment and software utilized for large molecule bioanalysis.- Mentors junior scientists through lunch & learn sessions and one-on-one job shadowing.- Bottom-up Applications: Antibody-drug conjugates (ADC), antibodies, biotherapeutics, biomarkers.- Top-down Applications: Utilize Q-Exactive series instrumentation to quantify intact biotherapeutics and biomarkers.Instrument expertise: --- Thermo Q-Exactive, Q-Exactive Plus, TSQ Quantiva, TSQ Vantage mass spectrometers.--- Sciex API 4000, 5000, 5500, 6500 --- Conventional flow Dionex Ultimate 3000 systems, Waters Acquity I-Class, Shimadzu Nexera and Prominence LC platforms. --- Nanoflow Dionex systems (2D, 3D).--- Hamilton STAR and Apricot Designs laboratory automation systems.

Durham, North Carolina, Us

- Post-doctoral research under mentorship of Dr. Jack Henion and Dr. Richard Abbott. - Developed a red blood cell filtration device capable of generating dried plasma spots from whole blood microsamples for use in quantitative bioanalysis.- Implemented high resolution accurate mass spectrometry (HRMS) instrumentation for detection and quantification of small molecules present in complex biological matrices. - Paired high-throughput dried matrix spot extraction technologies with HRMS and triple quadrupole mass analyzers.- Investigated alternative dried blood spot sample treatment workflows and card substrates to address analyte stability and blood hematocrit challenges.- Communicated developments of plasma microsampling strategy at the 2013 ASMS conference in Minneapolis, MN through oral and poster presentation mediums.Instrument expertise: --- Thermo Q-Exactive, Exactive Plus, TSQ Quantiva. --- Dionex and Shimadzu LC systems. --- Spark-Holland dried blood spot autosampler and Symbiosis online SPE systems.

Madison, Wi, Us

- Graduate research under mentorship of Dr. Lingjun Li. - Designed and implemented LC/MS, MS/MS based approaches to characterize and quantify small molecules, peptides, and proteins in complex biological matrices using multiple MS platforms.- Managed a small team junior graduate and undergraduate students.- Coordinated instrument installation, user trainings, and maintenance/troubleshooting.- Six public presentations.- Ten peer reviewed publications.- Thesis: Mass spectrometry method development for targeted and discovery neuroproteomics and peptidomics.Instrument expertise: --- QTOF: Waters micro and Synapt G2. Bruker maXis 4G.--- Ion-trap: Thermo LTQ, Bruker amaZon ETD.--- QQQ: AB Sciex 5500 QTRAP.--- TOF: AB Sciex 4800 MALDI TOF/TOF. Bruker AutoFlex III TOF/TOF.--- Waters Acquity and nanoAcquity UPLC.--- Waters Alliance HPLC.

Madison, Wi, Us

- Undergraduate and graduate instruction in laboratory and classroom environment.- Undergraduate instruction: General chemistry and advanced analytical chemistry.- Graduate instruction: Instrumental analysis.- Planned discussion and laboratory sessions.- Demonstrated laboratory safety.

Grand Rapids, Michigan, Us

- Research under mentorship of Dr. Elizabeth Jensen.- Developed method to extract and quantify flavonoid content in various varieties of Michigan red wines using analytical flow HPLC coupled to a diode-array detector.- Thesis: Quantification of trans-resveratrol and quercetin in a variety of Michigan red wines.

Grand Rapids, Michigan, Us

- Prepared media, slides, and plants for student labs. - Assisted in the upkeep of laboratory rooms and preparation areas.

- Research under mentorship of Dr. Elizabeth Jensen .- Co-authored competitively funded environmental chemistry research proposal.- Developed collection, extraction, and analysis protocols for measuring heavy metals in soil matrices.- Integrated GPS data into GIS software to graphically display sampling locations in rural and urban West Michigan locations.- Presented research findings orally at two public seminars in West Michigan.- Presented research as a poster at the Central Regional Meeting of the American Chemical Society in Covington, KY.- Research: Quantification of Pb, Zn, & Cu concentrations in various soil types located near heavily trafficked roadways and isolated areas free from vehicle traffic using atomic absorption spectroscopy.

- Performed environmental swabbing and QC testing for pathogens in food products.

Ph.D, Chemistry (Bioanalytical)

Bs, Biology, Chemistry