Rodrigo Guillen Email & Phone Number

Chapel Hill, Nc, Us

Durham, North Carolina, United States

- Immortalizing and genetically modifying primary mammary epithelial cells.- Characterization, maintenance and banking of genetically modified cell lines.- Studying the lactation phenotype of immortalized mammary epithelial cells.

Research Triangle Park

Supervisors: Kilsoo Jeon (PhD) & John Graef (PhD)• Maintained iPSC lines.• Differentiated iPSC lines for neural and cardiovascular research teams.• Developed a program for iPSC gene editing using CRISPR/Cas9

Chapel Hill, North Carolina, United States

Superviser: Jessica L Bowser, PhD. Assistant Professor, Department of Pathology and Laboratory Medicine

Nashville, Tennessee, United States

Supervisor: Kathy Gould, Ph.D. (Associate Dean for Biomedical Sciences, the Director of Graduate Student Support at Vanderbilt University School of Medicine, a Louise B. McGavock Professor, and Professor of Cell and Developmental Biology)Validating multiple candidate CK1δ/ε substrates. Collaborating with a colleague to produce CRISPR/Cas9-mediated point mutants in U2OS cells.

Nashville, Tennessee, United States

Supervisor: Kathy Gould, Ph.D. (Associate Dean for Biomedical Sciences, the Director of Graduate Student Support at Vanderbilt University School of Medicine, a Louise B. McGavock Professor, and Professor of Cell and Developmental Biology)Established and implemented a CRISPR/Cas9-mediated gene-editing protocol for the lab. Produced a catalog of putative substrates and interacting-partners of CK1δ and CK1ε through a combination of protein purifications and mass spectrometry analysis. Determined the full cohort and functional importance of CK1δ/ε-dependent phosphorylation sites on the endocytic protein GAPVD1. Described the consequences of CK1δ/ε inhibition on mitosis and DNA damage repair.

Supervisor: Nasser M. Rusan, Ph.D. (Senior Investigator, NHLBI, NIH)Design and implement experiments investigating the significance of calmodulin-binding motifs of multiple centrosomal protein. Maintained the labs fly stocks.

Chapel Hill, North Carolina, United States

Supervisor: Sabrina Burmeister, Ph.D. (Director of Faculty Diversity Initiatives)I analyzed protein expression of the transcriptional regulator Egr-1 using cross-sectional images of the posterior dorsal thalamus in female Rana p. pipiens after presentation with male mating calls.

Doctor Of Philosophy - Phd, Cell And Developmental Biology

Bachelor Of Science (B.Sc.), Psychology & Biology