Graduate research assistant under the Sagen Neurosurgery/Neuroscience Laboratory at the University of Miami: • Conducted precise sectioning of rat spinal cord tissues using cryostat machinery, ensuring consistency and quality for downstream analyses• Proficiently executed immunofluorescence staining techniques, contributing to the investigation of cellular markers and protein expression patterns• Performed Bicinchoninic Acid Assay (BCA) and Enzyme-Linked Immunosorbent Assay (ELISA), demonstrating proficiency in biochemical analysis methods• Operated advanced microscopy equipment including Olympus IX70 microscopes, facilitating detailed examination and imaging of tissue specimens• Conducted image analysis using ImageJ software, contributing to quantitative data extraction and interpretation for research projects

• Performed chart preparation per clinic protocol.• Reviewed completed charts with the provider in between patients. • Accompanied the provider to all scheduled patient visits. • Documented the patient history, physical exam, procedures, and patient plan as performed by the provider. • Entered radiographic and laboratory studies, as ordered by the provider. • Entered in medication and prescription orders as indicated by the provider.

• Conducted organization and preparation of diverse frog liver samples for quantitative Polymerase Chain Reactions (qPCRs) ensuring accuracy and adherence to experimental protocols• Facilitated DNA extraction from frog liver samples, employing meticulous attention to detail and adherence to laboratory safety procedures• Managed and organized extensive datasets using Excel sheets ensuring efficient data retrieval and analysis for research projectsFor more information on the lab, visit the website linked here: https://www.catenazzilab.org/