Rohan Acharya Email & Phone Number

Somerville, Massachusetts, Us

Currently working at bluebird bio in the Cellular Process Development team

Somerville, Massachusetts, Us

• Designing and performing experiments isolating and transducing CD34+ HSPCs• Executing and overseeing experiments optimizing process to isolate CD34+ HSPCs• Preparing and presenting packages showing experimental design, data and results• Writing reports on completed data packages

Somerville, Massachusetts, Us

• Designing and performing experiments isolating and transducing CD34+ HSPCs• Designing small scale model of CD34+ HSPC enrichment process• Preparing and presenting packages showing experimental design, data and results

Somerville, Massachusetts, Us

Worked on the Vector Process Development team to develop next gen viral vectors for gene therapy• Performing viral vector production experiments to assess the impact of process changes and/or new technologies• Mammalian cell culture experiments using bench scale and AMBR bioreactors, including media preparation, culture initiation, and seed train maintenance.• Performing assays to determine particle size and detect viral proteins in cell culture medium• Preparing and presenting packages showing experimental design, data and results

Lancaster, Pa, Us

Worked for Eurofins as a Upstream Process Development Scientist at a large pharmaceutical company:• Supported client's campaigns for upstream product development and commercialization• Media/Feed preparation and optimization studies • Use of various lab equipment including osmometer, blood-gas analyzer, nutrient metabolite analyzer and cell counter• Mammalian cell culture work using bench scale bioreactors and shake-flask• Ran weekly group meeting to review logistics going on within team and cascade announcements from Leadership• Assisted in assembling and inserting of cells and bacteria into the reactor for experimentation• Statistical analysis of experimental data using JMP• Prepared and presented packages showing experimental design, data and results• Prepared cell pellets for RNA extraction

Ithaca, Ny, Us

Throughout 2016 I worked in the lab simulating the small intestine using bioreactors. The purpose of the project was to develop an avenue for studying intestinal cells without using animal models or other less accurate in vitro models. Responsibilities and tasks included:• Created and modified bioreactors using CAD to be used in experiments involving intestinal cells and the effects of different bacteria on them. Bioreactors were used in place of live subjects to better understand and study cells. Scaffolds were also created for cells to grow on.• Created bioreactors that could be used with microscope to view cells without opening up reactor.• 3D printed numerous devices including created bioreactors for regular testing and experimentation• Assisted in assembling and inserting of cells and bacteria into the reactor for experimentation• Learned how to manage personal projects over a given time frame. Learned how to write and research a published work.

Ithaca, Ny, Us

In the summer of 2015 I worked in the lab dealing with waste water and its treatment at Cornell University. The purpose of the project was to investigate using granules as a method to clean wastewater and to test whether these reactors would be a better alternative to current cleaning methods.Responsibilities included:• Worked with Granular Sequencing Batch Reactors (GSBR) used to clean synthetic wastewater• Created reactors using instructions from a research paper. Timings were set using a program called Process Controller which was developed by a Cornell professor - this program had to be first learned before it could be applied.• Collected water samples from streams in the Ithaca area. Tested water using the Enterolert method to look for Enterococcus levels in the water.• Gained more experience in learning how to use new engineering tools with limited guidance. Learned independence and how to troubleshoot new equipment.

Ithaca, Ny, Us

In the summer of 2014 I worked in a research lab concerned with analyzing compounds secreted by cells and determining if they were harmful to the environment.• Analyzed Pseudomonas Putida cells for lab and the fluorescent compounds, or siderophores, that they secreted.• Gave bacteria various organic acids and varying amounts of iron needed to make the siderophores. • Analyzed the secreted compounds using a fluoroscope and presented the experiment to the professor and colleagues at the end of the summer.• Gained an appreciation for the type of work done in Biological Engineering and an increased motivation to do lab work in this field.

Ithaca, Ny, Us

Played trombone and marched at various sporting events. Was responsible for trombone section of 20 members. Assured that all members of the section were comfortable with music and marching as well and welcomed new members to the section. Organized section events and distributed music.

Ithaca, Ny, Us

Served food for hundreds of students, multiple times a week. Ensured good communication between full time workers and various chefs. Learned about different aspects of food service. Received a better appreciation for the food service industry and what they do. Because of the hectic schedule, developed good organizational and time management skills.

Worked as a student intern over the summer for several weeks. Main responsibility was working on cropping and formatting different images using Photoshop.

Master Of Engineering (M.Eng.), Biological And Environmental Engineering - December 2016

Bachelor Of Science (B.S.), Bioengineering And Biomedical Engineering - May 2016

High School Diploma