Rohan K. Email & Phone Number

Houston, Tx, Us

Houston, Texas, United States

I am working with Prof. Navin Varadarajan on multiple projects, including-Production of recombinant 2’3’-cGAMP from Escherichia coli• Designed complete upstream and downstream bioprocess for synthesis and purification of 2’3’-cGAMP, a potential new generation vaccine adjuvant.• Performed codon optimization using HTS-based methods and performed vector design and conjugation for protein expression.• Led bioprocess development from plasmid design, cloning and culturing (upstream) to and purification (downstream) processes. Performed bioprocess optimization resulting in significant yield improvement (~30%) over previous studies, resulting in patent application.• Performed intermediate characterization using RP-HPLC and LC-MS, as well as endotoxin testing for final product.Development of commensal bacterial strains for immunomodulatory response• Studied engraftment of engineered commensal bacterial strains producing immunomodulator in mouse model. • Collected biological samples (intestine) to study immune cell activation profiles in Flow cytometry setup.Development of intranasal vaccine for SARS-CoV-2• Designed, performed and analyzed experiments in molecular cloning, recombinant protein production (expiF/CHO-K1) and animal studies to study vaccine responses for a fusion antibody-based vaccine with mucosal delivery system to improve antigen presentation at site of infection in SARS-CoV-2.

Mumbai Area, India

Worked with Prof. Sarika Mehra and Dr. Vikas Chandrawanshi on the following project-Metabolic Flux Analysis (MFA) in CHO-K1 cells• Created a systems biology approach for studying metabolic network comprising major reaction pathways of cellular metabolites using growth and secretome data from Tunicamycin treated CHO cells.• Mapped both extra/intracellular reaction fluxes by bootstrapping and solving the overdetermined linear system of fluxes, showing variation between control and Tunicamycin treated CHO cells shows altered metabolism in particular pathways.

Mumbai Area, India

Working in Bio-similar Department at RLS in tandem with upcoming Novel technology facility project for Biosimilar production.

Varanasi Area, India

Completed of a 5 Yr. Integrated Dual Degree(Bachelors' in Technology and Masters' in Technology) in Biochemical Engineering & Biotechnology.

King Edward Memorial(K.E.M) Hospital, Elphinstone Road(East), Mumbai

Worked on a project regarding the Financial Feasibility of a facility for NPFC in Govandi, Mumbai (India), as well as a market report on therapeutic Blood products Prepared calculation charts and Batch Manufacturing Records for production of therapeutic Blood products like Factor VIII, Human Serum Albumin(HSA), Factor IX, Immunoglobulin G and Prothrombin Complex

Serum Institute Of India,Pune,India

Submitted a Project Report on the Quality Control Department,resulting in a Revision and Report on overall department dynamic & Standard Operating procedures for safety compliance.

Mumbai Area, India

Prepared a project report on the Engine overhaul and Maintenance Department, regarding General Engine Testing procedures and requirements.

Doctor Of Philosophy - Phd, Chemical Engineering

Admitted into the Chemical Engineering program at the University of Houston in Fall 2019.

Integrated Dual Degree, Biochemical Engineering And Biotechnology, 7.52/10

Activities and Societies: IIT(BHU) Quiz Club; Class Representative; Astronomy ClubCompleted and defended Masters' thesis titled.

Senior Secondary School, Sciences, 78%

High School, 87.69%

Activities and Societies: Debate Club Elocution Club Quiz Club