SKILLS & EXPERTISENext-Generation Sequencing Techniques• Multi-omics Profiling (10x Genomics)• Single-cell genomics / transcriptomics (Smart-Seq2/3; 10x Genomics)• Whole genome sequencing• Ribo-depleted RNA-Seq, Poly(A) RNA-Seq• RRBS-Seq, Single-Cell RRBS, Bisulfite Sequencing, CMS-IP• CUT&RUN, CUT&Tag, ChIP-Seq• ATAC-Seq, OmniATAC-Seq• NGS library preparationCell Biology• Cell culture (ESCs derivation, iPSCs generation, single-cell culture to generate ESC/iPSC lineages)• Adherent and suspension cell culture (Primary cells, cell lines, hybridoma production)• Cell transduction (Retroviral, Lentiviral), Transfection, Nucleofection/Electroporation• Magnetic cell sorting (MACS), Fluorescent-Activated Cell Sorting (FACS)• Multi-color flow cytometry• CyTOF mass cytometer HeliosBiochemistry / Molecular Biology• DNA cloning, Site-specific mutagenesis• DNA/RNA extraction/purification/analysis• Chromatin Immunoprecipitation (ChIP), Co-IP, Pull-down• Northern blot, Southern blot, Western blot• Radioactive EMSA• PCR, QPCR, RT-QPCR• Whole cell extract, Nuclear/Cytoplasmic protein extract• Recombinant protein extraction/purification (GST, MBP, 6xHis), Antibody purificationGenome Editing - Genetic Engineering• CRISPR-based genome-wide genetic screens + Multi-omics Profiling• CRISPR-Cas9 gene knockout and knockin• Transgenic mouse and cell line generation• Conditional gene targeting (Cre-LoxP, Flp-FRT)Microscopy• Immunofluorescence / Immunohistochemistry (human/murine cells, organs, and tissues)• Microtome-sectioning of paraffin-embedded tissues, Cryostat-sectioning of frozen tissues• Microscopes (Super-resolution Airyscan confocal laser scanning microscopy, Live-cell imaging, and Automated fluorescence microscopy)Animal Procedures• Animal handling, Time Matting, Injections• Fine Dissection, Necropsy• Controlled ovarian hyperstimulation (super-ovulation)• Mouse and Rabbit antibody production