Developed novel separation and recovery schemes for yeast and E.coli derived sesquiterpenes engineered on a synthetic biology platform.• Developed a novel, scalable aqueous extraction and precipitation method for separating Artemisinic Acid from yeast fermentation broth. I am co-inventor on a patent for this unique method. • Developed bench and scalable methods for separating Farnesene hydrocarbon from E.coli and yeast fermentation broths with >90% recovery yields. • Developed new assay and screening methods to predict downstream recovery potential in new strains.• Developed and implemented new in-process assays to monitor downstream recovery unit operations performance at pilot and manufacturing scales.• Designed experiments to characterize where desired products partition in process streams, from fermentation harvest to final crude product.• Designed and implemented experiments to determine mass balance yields across all downstream unit operations for both pilot and manufacturing scales.Developed methods for cleaning up and preparing various crude feedstocks for yeast fermentations. • Designed and implemented centrifugation, filtration and other methods for preparing raw cane and sorghum syrups and molasses.• Collaborated on a grant project with the U.S. Department of Energy to prepare and test various cellulosic derived feedstocks. These included hydrolysates of Loblolly Pine, Sorghum and wheat straw bagasse. I was involved in the scale-up and technology transfer of the downstream recovery process for the hydrocarbon Farnesene to multiple domestic and international manufacturing sites including Brazil, Canada, France and Spain. Recovery processes that I developed at the bench scale were successfully scaled up to fermentations as large as 300,000 L.

Utilized broad hands-on experience in the development of E.coli fermentation, protein recovery and isolation processes, protein folding, and supporting analytical techniques. * Developed a stable, post-refold precipitate intermediate that allowed de-coupling of refold and purification operations. This work explored protein precipitation using acids, a variety of salts and PEG.* Worked on the development of a high-density fermentation for proteins expressed in microbial hosts using a limited glucose feeding strategy to minimize cytotoxic acetate production. * Developed stable recovery processes for proteins expressed in microbial and mammalian hosts. Projects included: - Determining and optimizing process chemistry. - Determining optimal equipment and environmental operational parameters, including range finding and determination * Produced small-scale GMP manufacturing lots to support clinical trials of both licensed and early stage products. * Developed process and equipment improvements for a large-scale solvent extraction recovery utilizing a centrifugal three-phase separator.Involved in the Fermentation/Recovery scale-up, technology transfer and validation of several commercial and clinical products expressed in E. coli and Pichia. I Provided commercial manufacturing support for the licensed products PDGF, IL-2 and IFN-β. I worked on joint projects with Pfizer, Entremed, Alexion and Zymequest.

*Assisted in the development of recovery processes for proteins expressed in microbial and mammalian hosts. Projects included TFPI (stable recovery operations/optimal chelating agents), IFN-β (high throughput upstream operations), IL-2 (reduced oxidation during recovery operations), *Assisted in the development of fermentation processes for proteins expressed in microbial hosts. Projects included Azyme (β-Galactosidase) high-density fermentation development (including limited glucose feeding strategy, minimizing cytotoxic acetate production)*Mammalian cell recovery projects including tangential flow and “Dead-End” filtration recovery/separation of mammalian cells. *Produce small scale manufacturing lots to support clinical trials. Projects supported included IFN- β, IL-2, Bench scale experience in shake flask & fermentor culture, recovery techniques and associated analytical methods.Scale-up and technology transfer and validation of processes to 1,500 liter scale (Emeryville) and 12,000 liter scale (Vacaville)*Technical assistant/contact for scale-up and transfer of IFN-β fermentation & recovery operations from 1,500 to 12,000 L scale in Vacaville. Optimization of upstream recovery operations for best facility fit. *Technical lead for recovery in the scale-up and transfer of TFPI fermentation & recovery operations from 10 to 12,000 L scale in Vacaville.*Technical lead for the validation of recovery operations for the manufacture of TFPI in Vacaville.*Authored Process Validation Protocols and summary reports for recovery operations for the manufacture of TFPI in Vacaville.

Operated and maintained 10 liter Chemap fermentors and proprietary GroBug control system. Prepared media, buffers solutions, and build-up and seed innoculum. Analyzed nutrients and analytes during the progression of fermentations using YSI and Kodak Biolyzer instruments. Performed harvest and recovery procedures. Operated and maintained Sartorius tangential flow filtration system. Assisted in operation of 100 liter fermentor, continuous flow centrifuge, and homogenizer. GMP work experience. Input and analyzed data using Microsoft Excel and Word.