Ronald Nguyen Email and Phone Number

PI: Wendy LiuMentor: Sergei ButenkoProject: Optimizing Wound Healing Efficiency and Mechanisms With The Applications of Biomaterial Cell Culture Experience: 3T3, CHO, RAW 264.7, Primary Fibroblasts, Bone Marrow-Derived Macrophages, Jurkat• Carried out RNA extraction, purification, cDNA synthesis, and quantitative PCR (qPCR) to support gene expression studies • Performed qPCR analysis on several markers to assess for fibroblast activation (myofibroblasts) (Col1a1, Tagln, Acta2) & Tnfsf11/RANKL expression• Experience in microtome sectioning and histological staining (Masson's Trichrome, H&E)• Conducted mouse genotyping using TransnetYX to optimize breeding in addition to harvesting primary cells • Mentored incoming undergraduate students, providing hands-on guidance in experimental protocols and harvesting techniques, fostering research engagementPresentations:Edwards Summer Undergraduate Research Program (ESURP): Poster Session (2023)Edwards Summer Undergraduate Research Program (ESURP): Poster Session (2024)

PI: Wendy LiuMentor: Daniela Enriquez OchoaProject: Investigating Macrophage-Fibroblast Dependent Crosstalk in the Foreign Body Response• Fabricated polyacrylamide gels as a substrate for an in-vitro study utilizing bone-marrow derived macrophages, dermal fibroblasts, and cytokines to test its effect on macrophage fusion based on analysis in-vivo• Refined primary cell protocols, cell line culture protocols, harvest protocols, and designed experiments• Conducted FIJI (ImageJ) analysis on fusion levels from images taken on FV3000 laser scanning confocal microscope

PI: Naomi Chesler• Inventory Control of Cell Culture Supplies, Cryopreservation Supplies, Liquid Nitrogen, Carbon Dioxide Tanks, and Nitrogen Tanks• Maintenance of Hoods, Benches, Dewars, Incubators, Nitrogen Line, Carbon Dioxide Levels, Vacuum Lines, and Microscopes• Established Seamless Communication Between Lab Space and Management to Meet Needs and Orders• Enforced EHS Safety Guidelines • Maintained Cleanliness of the Shared Space

PI: Abraham LeeProject: Optimizing and Characterizing Synthesis of Senescent T cells• Reviewed current practices of senescent & exhausted T-cell experimentation and analysis• Conducted review of markers and targets for senescent & exhausted T-cells • Planned and revised experiments to induce stresses to human T-cells isolated from whole blood to prompt the synthesis of nonfunctional T-cells w/ flow cytometry confirmation

PI: Abraham LeeMentor: Mohammad AghaamooProject: High-Throughput Acoustic-Electric Shear Orbiting Poration (AESOP) Platform for Intracellular Delivery of Cargos• Utilized SolidWorks to design different arrays of piezoelectric transducers to facilitate acoustic streaming and turbulent flow for the application of mixing and mechanical shearing of cells for the application of CAR-T cell therapy• Fabricated patterned polydimethylsiloxane (PDMS) microfluidic chips and soldered piezoelectric transducers for testing w/ a waveform generator • Measured impedances of piezoelectric transducers to maximize the output power of ultrasonic waves on a substrate w/ Zurich Instruments HF2LI Lock-in Amplifier• Characterized vortex arrays and noted regions of no activity for optimizationPresentations:CADMIM Summer Showcase: Poster & Oral Session (2023)CADMIM Industrial Advisory Board Meeting (IAB) Showcase: Poster Session (2024)ERN/REM Conference: Poster Session (2024)

Electrical Mentor:• Held sessions teaching students how to solder, crimp, and apply Anderson connections• Assisted in the design of the electrical system w/ high school students• Wrote an Arduino program to activate a color sequence from an LED strip depending on the mode the robot was in• Overlooked two high school students on the final design of the electrical system and as well as workflow.• Assisted in improving the OC Pit Scouting Document for an easier viewing experience.

• Fabricated a photosensitive sensor to facilitate the climbing process• Optimized the tubing path and wired the pneumatics for the arm of the robot• Soldered connections and worked with the mechanical team to ensure organization.• Repaired and adjusted the design of wire management over time for qualification as well as organization in locating issues with hardware over time.• Analyzed and communicated with allying robotics teams to strategize to exploit weaknesses of opposing robots for a higher chance of winning matches.

• Managed finances for in-store and online sales, improving customer experience through bilingual recommendations in English and Vietnamese.• Updated online website weekly for promotions, sales, and inventory, enhancing user engagement and driving sales growth.• Handled order fulfillment and inventory management weekly, ensuring efficient operations.

• Sold 384 items worth over 10,000 dollars across the United States w/ Top Seller Status• Tracked Wholesale Prices of Beauty Products and Maintained Sales Within Competitive Market• Managed Finances, Shipping, Customer Inquiries, and Inventory• Obtained and Sustained High Customer Satisfaction Ratings