Microbiological testing of pharmaceutical row materials, in-process materials, intermediates and final products under Ph. Eur. specifications and GMP guidelines. Routine microbiological assay and techniques as described in appropriate SOPs and work procedures (aseptic testing, microbial monitoring, sub culturing, plating, microbial and samples characterization and identification, media and solutions preparation and validation). Writing protocols, performing assay validation and providing technical guidance. Laboratory equipment qualification/validation. Reviewing IQ/OQ/PQ protocols. Drafting QC documents, GLP, GMP, HACCP procedures. Cooperation with QA in investigations/CAPAs/OOS/OOT. Interaction with QA and regulatory agencies during audits. Environmental monitoring evaluation (air, surfaces, water, steam). Counter samples and reference materials management (sampling, NIR spectroscopy identification, approval or rejection). Use and management of Data Loggers. Performing Logbooks reviews. Laboratory waste and management. Planning and Performing non-routine method and procedures. Evaluating lab practices for compliance and operational improvement.

Microbiological research applied to the development of probiotic-based product and their production scale-up. Selection and characterization of probiotic strains. Preliminary and feasibility studies for the development of new products: combination of new active ingredients or excipients, evaluation of the interference between probiotic strains and new potential substances as well as symbiotic effect. Analysis of the optimal conditions in the fermentation and bacterial freeze-drying processes. Microbiological analysis on medicinal specialties and food supplements.Stability studies on the finished product (freeze-dried) and on the other Proge Farm products. Preparation of related protocols and reports and statistical data analysis.Bacterial genetic characterization (RAPD, ARDRA, MULTIPLEX, SSCP, PFGE, REP, ITS, RT-PCR) and phenotypic characterization(API, BIOLOG).Development of a method for determining the adhesion of bacterial cells to the vaginal epithelium (ex vivo) and the consequent formation of Biofilms.Evaluation of the resistance of bacterial strains to the gastrointestinal tract by in vitro techniques. Antibiotic Resistance Studies.The company experience, at the same time, included a training period in which I carried out a PhD aimed to identifying and characterizing functional bioactives with prebiotic activity isolated from food matrices and by-products, with consequent evaluation of their effect in the fermentation of microorganism as new potential functional foods. Main analytical skills: antioxidant activity (DPPH, ABTS), Kjeldahl, Folin-Ciocalteau, TDF determination (AOAC method), β-glucan determination, HPLC-DAD-MS, various electrophoretic technologies (agarose, polyacrylamide, SDS PAGE, UREA PAGE, two-dimensional), protein purification techniques.

Oligosaccharides characterization and identification. Main analytical skills: HRGC/FID, Q-TOF MS, MALDI-FTICR