Research Technician I
Chevy Chase, Maryland, Us
Howard Hughes Medical Institute Research Technician in department of Molecular Pathogenesis at NYU/Skirball Institute of Biomolecular Medicine. Researching new subset of helper T cells (Th17) that up-regulate a pro-inflammatory cytokine (IL-17)) involved in multiple autoimmune diseases.Zhou L., Ivanov I.I., Spolski R., Min R., Shenderov K., Egawa, T., Levy D.E., Leonard W.J., Littman D.R. “IL-6 programs T(H)-17 cell differentiation by promoting sequential engagement of the IL-21 and IL-23 pathways.” Nat Immunol. 2007. 8(9):967-74.Zhou L, Lopes JE, Chong MM, Ivanov II, Min R, Victora GD, Shen Y, Du J, Rubtsov YP, Rudensky AY, Ziegler SF, Littman DR. "TGF-beta-induced Foxp3 inhibits T(H)17 cell differentiation by antagonizing RORgammat function." Nature. 2008 May 8;453(7192):236-40. Epub 2008 Mar 26.• Trained on applications for flow cytometry, laser capture microdissection, confocal microscopy, immunohistochemistry/immunofluorescence, real-time PCR, Western blotting, co-immunoprecipitation• Tissue culture work with purified naïve CD4 lymphocytes transduced with a retrovirus, treated with various cytokines, and performed intracellular staining for analysis• Handled transgenic mice and performed embryo isolations, in vitro fertilizations and rederivations• Worked closely with post-docs on complex projects for my masters thesis and won first place for poster presentation