Rúben Salvado Email & Phone Number

Lisbon, Portugal

Fundão, Castelo Branco, Portugal

Multiple Angular projects for different companies.ASP.NET MVC project for a bank.

Fundão, Castelo Branco, Portugal

Development of webapp for automotive Infotainment. The webapp was developed with Angular Framework, using TypeScript.

Coimbra E Região, Portugal

Thesis title: “Oxidative Stress Biomarkers - Closer to early diagnosis of neurodegenerative diseases?”Accomplishments (expected)- Detect and characterize possible biomarkers for Parkinson’s Disease using an HPLC-MS/MS approach.- Deepen the knowledge of the effects of oxidative stress in the metabolome of neurodegenerative diseases.Techniques- HPLC-MS/MS (Triple TOF 5600)- Statistical Analysis (MarkerView, SPSS)- Sample preparation techniques

Portugal

Thesis Title: “Mitochondrial-targeted ability of Plasmid DNA Vectors”Accomplishments- Successful development of plasmid DNA nanoparticles with ability to target the mitochondria- Demonstration of this ability in different cell lines with use of confocal microscopy- Development of a new plasmid containing the mitochondrial gene ND1 in pGEM®-T- Published results in a peer reviewed journal (DOI:10.1016/j.colsurfa.2014.12.013)Techniques used- Microbiology techniques (culture and transformation of bacteria, fermentation and media preparation)- Amplification and purifications of plasmids and genes (PCR)- Sequencing and electrophoresis of nucleic acids- Cell line culture- Confocal Microscopy and SEM

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