Ruth Bauer, M.S. Ascp(Mb) Email & Phone Number

United States

Burlington, Ma, Us

Led the research and evaluation of 12+ companies with emergent technologies, integrating 5 technologies into a new device and new IP, driving innovation and expansion of the company's portfolio.Researched and tested 100+ combinations of excipients for assay lyophilization and rehydration leveraging deep knowledge of molecular biology, chemistry formulation and analysis.Prepared highly organized reports, implemented an internal tracking system for planning, execution and analysis and recording of experimentation. Presented as SME to international and domestic teams.Formulated PCR master mix using a multipronged screening for 30 enhancers and 20 enzymes, resulting in flexible, ultra-rapid PCR (<15 minutes) chemistry.

London, Gb

✤ Supervised and mentored two high-performing research teams consisting of six direct reports as they collaborated on several large projects while managing the clinical development and feasibility testing of the medical device product. ✤ Utilized LiDiaTM system to screen and detect a panel of over 30 blood pathogens and antimicrobial resistance markers (AMR).✤ Formulated and allocated annual budgets, identifying essential personnel, CAPEX and non-CAPEX equipment, and service contracts needed for the department. ✤ Shared testing results with all team members and gathered findings into comprehensive reports to support all design process decisions made by executive management.✤ Negotiated and finalized terms of the contract that enabled the continued delivery of lyophilization materials companywide. ✤ Motivated and inspired cross-functional teams comprised of manufacturing, quality, product integration, and R&D members to attain the successful integration of workflow into a developed automated medical device, thus boosting overall efficiency over 100%. ✤ Partnered with executive management, business development, and R&D teams to assess project needs, determine scientific direction, and ensure milestone achievement.

London, Gb

✤ Created and implemented a multiplexed model ‘6-plex’ qPCR assay tool that promptly assessed pH-PCR chemistry and overall performance of the pathogen capture system, while increasing process productivity.✤ Ensured compliance with all CDC requirements, applied approved permits to all purchases and maintained inventory for the blood pathogen bank containing hundreds of research microorganisms (level BSL-1/2). ✤ Applied for USDA plant pathogen permit for use as an internal control on a highly multiplexed molecular platform.✤ Devised and oversaw molecular migration procedures to QA/QC team for implementation including PCR swab tests and DNA plasmid preparation. ✤ Analyzed 20 molecular assays, a ’20-plex’, to identify and promptly resolve such issues as human DNA interference reduction using nuclease, qPCR optimization, DNA isolation and end to end performance. ✤ Calculated mass balance of process to determine total loss of sample at each step which resulted in an 50-90% improvement in overall sample yield. ✤ Evaluated 5 DNA isolation methodologies for assay compatibility and analyzed data to make critical recommendations for the commercialization of the final product.✤ Spearheaded efforts to gain additional lab space and three Core Lab personnel, supervising the newly formed laboratory and earning a 10-fold throughput increase across all technical teams.

London, Gb

✤ Oversaw all aspects of molecular assay development, including Taqman® probe-based and nested PCR, to target blood pathogens. ✤ Validated an in-house fungal multiplex ‘5-plex’ qPCR assay for use with Candida species targeted capture system. ✤ Collaborated with 20 team members over three years on $21.5M BARDA government contract to develop an innovative product that enabled Bacillus anthracis to be detected directly from blood. ✤ Leveraged knowledge of molecular DNA isolation to create six ‘DNAfree’ reagents and consumables for efficient pathogen DNA extraction. ✤ Submitted patent idea on the removal of DNA from the chaotropic reagent used in the extraction process.

Vista, California, Us

✤ Confirmed Inflammatory Bowel Disease (IBD) and Irritable Bowel Syndrome (IBS) lab developed gene expression real-time PCR assays for CLIA lab. ✤ Isolated RNA using automated Qiagen QiaCube, RT-PCR, and LDT above on ABI 7300 real-time PCR instrument. ✤ Performed 100+/month clinical tests with 3 PCR assays using automated qPCR plate setup instrument (Eppendorf EpMotion 5070) and Real-Time PCR instruments (Applied Biosystems 7300, 7500).

Albuquerque, Nm, Us

✤ Conducted 500+/month clinical patient samples on multiple platforms and instruments to screen for genetic testing or acquired mutations in Molecular Diagnostics department. ✤ Provided ongoing assistance to two senior scientists as the lab introduced two additional esoteric testing validations for colorectal tumors and blood clotting polymorphism detection. ✤ Participated in annual CAP and CLIA accreditation and earned the Presidential Award for H1N1 support to virology during 2009 outbreak. ✤ Facilitated molecular esoteric testing including: BCR-ABL, PML-RARA, Factor II/V, IVGH, JAK2, CTFR, MGMT. ✤ Worked with 5 medical directors to analyze data and obtain results that delivered the best-care to clinical patients.

Vista, California, Us

✤ Contributed to the research and development of two FDA submitted studies on breast cancer prognostic kit. ✤ Specialized in transferring technology to the manufacturing department and optimizing molecular assays. ✤ Participated in the creation of assays and diagnostics tests via fluorescent in-situ hybridization (FISH) and quantitative PCR techniques, including estrogen receptor negative breast cancer and Hepatitis C virus inflammatory response. ✤ Managed laboratory staffs of five or more scientists during projects that lasted for one month, per the request of executive management. ✤ Handled, organized, tracked, and inventoried large sample database and specimens upon receipt for use with various studies.

Dallas, Texas, Us

✤ Studied cell division and developed a new protocol to view larval fly brain cell development using FISH techniques.✤ Ran 50+ experiments on centrosomal protein Centrosomin (Cnn) to test for the biological functions of in vitro purified proteins. ✤ Researched chromosome segregation in Cnn mutants genetically and by FISH procedures.

Dallas, Texas, Us

 Garnered basic laboratory skills while performing entry-level fruit fly laboratory support work.

Masters Of Science, Genetics And Development

Bachelor Of Science - Bs, Biology/Biological Sciences, General