Ryan Kyger work email
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Ryan Kyger personal email
I am a bioinformatics & data scientist. I have experience programming in R, Python, Linux (bash), SQL, C++. I have experience doing next generation sequencing (NGS) data analysis (variant calling, RNAseq, ATACseq, WGS, WGBS, Illumina, Nanopore, PacBio). I have experience with Frequentist & Bayesian statistical methods. I have molecular biology laboratory & experimental design experience. I have an accredited business management certification. I am capable of working independently or collaboratively in functional & cross-functional teams.
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Data ScientistAmgen Mar 2024 - PresentThousand Oaks, Ca, Us -
Associate Scientist (Contracted Via Donatech)Amgen Jun 2021 - Mar 2024Thousand Oaks, Ca, Us• Completed genomic DNA sequence variant analysis using fastp, bwa, GATK, octopus, samtools, bcftools, SnpEff, shinyGO on Illumina short-read whole genome sequence (WGS) data from different CHO cell lines on an AWS EC2 Linux instance to measure genetic differences between the cell lines. • Completed genomic DNA sequence variant analysis using fastp, pbmm2, GATK, pbsv on Pacific Biosciences (PacBio) HiFi long-read whole genome sequence (WGS) data from different CHO cell lines on an AWS EC2 Linux instance to measure genetic differences between the cell lines. • Completed differential expression analysis using a nextflow nfcore rnaseq pipeline (FastQC, STAR, stringtie, salmon), DESeq2, pathfindR on bulk RNAseq data (Illumina) from different CHO cell lines on an AWS EC2 Linux instance to measure mRNA expression differences between the cell lines.• Completed ATACseq analysis of CHO cells using a nextflow nfcore atacseq pipeline, HMMRATAC on an AWS EC2 Linux instance. • Completed Oxford Nanopore long-read structural variant calling using pycoQC, minimap2, NGMLR, SVIM, sniffles, cuteSV on an AWS EC2 Linux instance. • Collaborated with team members to develop an automated variant calling pipeline using fastp, bwa, GATK, octopus, lofreq, samtools, bcftools, SnpEff, that runs on AWS EC2 Linux instances, for protein quality control.• Collaborated with cross-functional team members to develop machine learning models to predict high protein producing CHO cell clones.• Completed de novo assembly of DNA sequences of proteins using MEGAHIT, Flye. • Created heatmaps of Nanostring nCounter gene expression data using R. • Developed an R script that outputs an interactive visualization & regression of mutation frequency vs time data.• Documented code in company GitLab repositories. -
Phd Student (Cell & Molecular Biology)University Of Nevada, Reno Aug 2018 - Feb 2020Reno, Nv, Us• Published discovery that myxozoans (parasitic jellyfish) lack cytosine DNA methylation, by analyzing Whole Genome Bisulfite Sequence (WGBS) data (DOI: http://dx.doi.org/10.1093/molbev/msaa214). -
Master’S StudentSan Francisco State University Aug 2015 - Jun 2017San Francisco, Ca, Us• Investigated the Endoplasmic Reticulum in D. melanogaster embryos, using molecular biology & fluorescence microscopy. -
Undergraduate Student & Laboratory AssistantUniversity Of California, Davis Jun 2014 - Jul 2015Davis, California, Us• Investigated Kinesin-5 motor proteins in S. cerevisiae, using molecular biology, biochemistry, & fluorescence microscopy.
Ryan Kyger Skills
Ryan Kyger Education Details
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San Francisco State UniversityCell/Cellular And Molecular Biology -
Uc Riverside ExtensionBusiness Management -
Uc San Diego Extended StudiesApplied Bioinformatics -
University Of California, DavisCell/Cellular And Molecular Biology
Frequently Asked Questions about Ryan Kyger
What company does Ryan Kyger work for?
Ryan Kyger works for Amgen
What is Ryan Kyger's role at the current company?
Ryan Kyger's current role is Bioinformatics, Data Science.
What is Ryan Kyger's email address?
Ryan Kyger's email address is rk****@****gen.com
What schools did Ryan Kyger attend?
Ryan Kyger attended San Francisco State University, Uc Riverside Extension, Uc San Diego Extended Studies, University Of California, Davis.
What skills is Ryan Kyger known for?
Ryan Kyger has skills like Spyder Ide, Bioconductor, R Shiny, Rnaseq Analysis, R, Git, Public Speaking, Linux, Microsoft Onenote, Sequence Alignment, Microsoft Powerpoint, Computer Hardware Assembly.
Who are Ryan Kyger's colleagues?
Ryan Kyger's colleagues are Adam Biddlecome, Ana Clary, David Scacco, Ao Santiwong, Sean Santillano, Ken Johnson, Galin Spicer.
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