Ryan Ruf Email & Phone Number

De Pere, Wi, Us

Green Bay, Wisconsin

Waunakee, Wi

•Perform DNA extractions and PCR/qPCR analysis of routine Heparin samples.•Test raw materials, In-process samples, and finished product Pancreatin/ Heparin for microbiological contamination per SOP.•Training of new analysts•Perform routine environmental sampling throughout the facility, which includes weekly/monthly water and air sampling.•Experience writing and executing studies/protocols.•Execute cleaning validation sampling and testing on production equipment.•Perform monthly and quarterly cleanings and calibrations of laboratory equipment.•Experience working within various types of Biological Safety Cabinets/Hoods.•Knowledgeable with the use of spectrophotometers and thermal cyclers.•Communicates with and works closely with other members of the lab.•Familiar with cGMP protocols, data recording, documentation, and safety procedures.

Madison, Wi

•Successfully cloned several genes of interest in both a feeding RNAi vector and GFP vector for embryonic lethality and localization studies in C. elegans.•Performed various molecular biology techniques including western blots, DNA gels/blots, PCR reactions, transformations, minipreps, restriction enzyme reactions, ligation reactions.•Worked as a work study student for a semester preparing solutions, pouring plates, cleaning laboratory equipment, autoclaving solutions and equipment, etc. •Accustomed with aseptic technique.•Conducted experiments for several genes of interest pertaining to cytokinesis.•Maintained several worm lines as necessary for experiments.•Conducted lab meetings to report on my research progress and answer questions.

Bachelor Of Science - Genetics, Genetics

Activities and Societies: Wisconsin Collegiate Bowling Conference- President 2008-2009

Education record