Ryan Mease Email & Phone Number

Denver Metropolitan Area

Greater Denver Area

-Plans and performs product demonstrations at customer sites-Participates in the field test site determination and/or execution process.-Represents the Life Science Solutions group of ThermoFisher Scientific at conferences and vendor shows to present products and customer application feedback.-Work with (and independently) Technical Specialists and/or Sales Representatives on sales calls in customers' laboratories to provide technical and applications assistance in response to customer inquiries.-Supports conditional sales placements in the field through on-site visits and telephone communication.-Provides start-up applications training to customers in their laboratories-Provides troubleshooting support to customers in the field or by phone/email.-Provides consultative support to existing and experienced customers-Resolves and/or escalates customer issues & complaints.

Greater Denver Area

•Oversees personnel performing daily high complexity and high-throughput testing and provides onsite or remote support and guidance while testing is performed •Monitors Illumina testing equipment, test analysis and specimen examination to ensure that acceptable levels of analytic performance and operational quality are maintained•Resolves technical problems, performs troubleshooting with library preparation and next-gen sequencing and ensures corrective actions are properly documented according to CLIA regulatory standards•Monitors personnel training and makes sure all training records are properly documented•Works to manage laboratory work force to maximize efficiency and reduce clinical laboratory operating costs•Assists in laboratory scale-up with equipment to produce an efficient, high-throughput laboratory workflow•Annually evaluates Quality Control and Quality Assurance and documents the performance of all testing personnel as required.•Assists the Quality Assurance department in the completion of workplace inspections and process audits•Generates or reviews current standard operating procedures to reflect high-throughput and high-complexity testing

Gaithersburg, Maryland

•Manages daily operations of all Illumina sequencing systems at GeneDx. Ensures all instruments are running at optimal levels in regards to capacity and data quality.•Manages daily/weekly/ run scheduling and setup of all sequencing systems according to established SOPs for clinical sample sequencing.•Manages sequencing technicians, in particular documenting quality control of sequencers and sequencing data according to CAP regulations. Documents all corrective action or deviations when needed.•Monitors operations and identifies problems with laboratory instruments and act as a liason with Field Service engineers and Technical Support staff.•Establishes standing orders for sequencing reagents so that the sequencing lab is fully operational.•Updates clinical laboratory SOPs and laboratory policies to reflect changes in sequencing workflows or reagents.•General tasks include coordinating of daily tech schedules, weekend scheduling, troubleshooting bottlenecks that could lead to disruption and output of clinical sequencing.

Gaithersburg, Maryland

-Supervises daily operations and scheduling of testing personnel following established standard operating procedures for specimen handling and processing, test analysis, reporting of results to team leads, test managers, and record-keeping.- Ensure that all group members adhere to quality control policies and document all quality control activities, instrument and procedural calibrations, reagent preparation and testing, and instrument maintenance performed.-Identify problems that may adversely affect test performance, take authorized corrective action, and notify key individuals. -Detects when test performance is not within acceptable levels. Documents all corrective action.-Always be able to document that proficiency testing is performed in the same manner as patient testing.-Meets or exceeds target workload with high accuracy and attention to detail. -Ensures that all technicians adhere to quality control policies and monitors all quality control documents, instrument and procedural calibrations, reagent preparation and testing, and instrument maintenance-Specific tasks include: coordinating daily schedules, distribution of work and usage of laboratory resources, continuous monitoring of daily test performance, identifying and troubleshooting problems that may adversely affect lab workflow or test results,monitoring bottlenecks and issues in operations and devises and tests solutions and improvements, contributing to creating and maintaining SOPs-Supervises training and competency testing of technicians, ensuring training records are completed, inventory and ordering of supplies.

Gaithersburg, Maryland

•Produce and analyze data from a wide variety of laboratory techniques in the BioServ department. •Serving as Illumina next-generation sequencing platform lead -Library preparation, exome sequencing via NimbleGen, Agilent SureSelect kits, RNA- Seq, DNA-Seq, cluster generation, Illumina HiSeq 2000 sequencer loading/monitoring and run scheduling - Clinical sample handling and preparation for sequencing in CLIA-certified lab, project scheduling and prioritizing•Served as interim laboratory manager (November 2012- February 2013) in addition to Illumina platform lead -Compiled weekly lab schedule, had oversight of day-to-day and weekly lab operations & projects -Worked with Edge project managers to prioritize incoming lab projects -Responsible for ordering of all laboratory reagents and monitoring purchase order history•Sample QC, sample processing of client DNA samples for Sanger sequencing using AB 3730xl, maintaining CE reagent supply inventory•Experience with Next Generation Sequencing-SOLiD4 and Ion Torrent sequencing platforms:DNA fragment library preparation, DNA mate-paired library preparation, Manual/automated ePCR, manual/automated sample enrichment, Sample deposition and reagent preparations, maintenance, operation, and managing supply inventory for both SOLiD4 and Ion Torrent sequencers•RNA sample QC, and sequencing using Applied Biosystems and NuGen RNA-Seq protocols•Operation of automated Next generation sequencing workflows and sample processing using the Caliper Sciclone NGS workstation•Assist in writing and editing of laboratory SOPs for general research and CLIA-approved protocols•Training of new employees in Illumina and Ion Torrent laboratory protocols, standard lab sample QC procedures and daily lab operating procedures

Silver Spring, Maryland

Malaria Vaccine DevelopmentCharacterized animal and human sera and proteins by ELISA, or Western Blot PCR and DNA cloning of malarial antigens in E.coli to express and purify such proteins as reagents for immunization in animal vaccine studies or laboratory reagentsEvaluated concentration of bacterial endotoxin levels in vaccine candidate antigensOptimized expression and purification conditions using GMP SOPs for vaccine candidate antigens for later development in WRAIR GMP production facilities (PfCelTOS)Quantified concentrations of proteins and specific antibodies in solution for subsequent experimentsOptimized conditions for functional assays that evaluate biological activities of immune sera from participants of clinical trials, as well as individuals from malaria endemic areas of Africa.Developed high-throughput methods for the preparation and purification of immunoglobulins from sera obtained from individuals in malaria endemic areasPlayed a crucial role in the evaluation of various clinical trials by ELISA with small volume samples from pediatric and adult individuals from Kenya and Mali (MAL036)Responsible for maintaining equipment service records and scheduling equipment service appointmentsResponsible for keeping stock of supplies Analyzed, prepared data and help write publications and poster presentations at conferences

Hagerstown, Md

Helped maintain and catalog blood and tissue samples for patients involved in ClueII Cancer studyEvaluated and cataloged patient questionnaires and data to determine epidemiology of cancer in surrounding areaUsed epidemiological data to assist in evaluating methods of early detection, and monitoring health behaviors and outcomes in the community

Performed DNA purification and restriction analysis of both bacterial plasmid and bacteriophage DNA samplesConducted bacterial growth experiments in the presence of various phage to identify strain virulence and optimize growth experiments Performed DNA cloning to incorporate bacterial and bacteriophage lysogen into same plasmid. Cloned combined plasmid into E.coli .Prepared results of experiments for presentation to members of the department and internship program