Ryan Johnson Email & Phone Number

Managing a Toxicology and Molecular lab in addition to being responsible for Molecular. Brought expertise to make Lab CLIA compliant and operations to run smoothly. Lab Manager and Molecular Technical Supervisor. Order supplies, explain results, plan with sales-team, upkeep and maintenance of Kingfisher and Quant12 studio, stability studies.

• Extract samples for RNA/DNA analysis using Real-Time PCR, Covid and RPP
• Luminex Mag-Pix system, DETECTX, PATHOGENX, Hamilton Nimbus, AB Thermal cyclers
• Explain results and sources of contamination. Biochemistry techniques.
• Advisement on new techniques and instrumentation
• Some hematology, toxicology, MassHunter.
• Quality Control and Quality Assurance. Validations and LODs.

Part-time online instructor for Organic Chemistry 2.

• Quality Control and Quality Assurance.
• Pathogen panels (extracted DNA and RNA) on Quant 12 and Quant 3 (Quantitative Polymerase Chain Reaction)
• Complete Blood Counts and drug screening (Indigo).
• Supervised people in my area and trained other employees on techniques.
• Laboratory Information System (LIMS)

• 6 Week Teaching Session.
• Taught General Chemistry, Math and Clinical Chemistry with lab.
• Encouraged proper technique and safety.

• Enzyme-Linked Immunoabsorbent Assay (ELISA) on clinical samples (Chemiluminescence with Biotek).
• Quality Assurance using gas chromatography mass spectrometry (GCMS) and viscometry.
• Western Blot/Native gels on harvested antibodies.
• Purification of antibodies for ELISA plate/method development.
• Rate experiments on new drugs using isotope P32. Receipt/Tracking/Disposal of P32.
• Reverse Transcriptase Polymerase Chain Reaction (RTPCR), Ultraviolet-Visible light (UV-VIS), FTIR, spectrophotometry

Pittsburgh, PA, US

• Answering questions and solving problems from Engineers and other Scientists.
• Operated two STA429s (Simultaneous Thermal Analysis), two Netzsch dilatometers, one Theta dilatometer and a STA449 with Thermal Gravimetric Analysis/Differential Scanning Calorimetry/Mass Spectrometry (TGA/DSC/MS).
• Method development, LIMS and some particle-size distribution.
• Responsible for group safety. Safety measures such as lockout/tag out.

Moon Township, Pennsylvania, US

• Chemistry I lecture and Physics lab. LIMS.
• Taught the basic terminology, problem solving skills, proper techniques and independent thinking.
• Prepared and presented lectures, quizzes, tests, score and report grades.
• Found importance to present material in additional ways to help students quickly grasp the important material.
• Responsible for safety of students and supervision.
• Shared different ways to think about things to allow not only better understanding but to easily connect to higher concepts.

Pittsburgh, PA, US

• Cell culture (MS2, Vero, MS-2), measuring titers, p24 antigens and drug inhibition experiments with cell cultures, HIV inhibitor drug screening and IC50 using Fluorescence and 3H.
• Research in Pathology was focused on detecting microRNA in prostate cancer tissue.
• Lavaged mice and harvested lungs for flow cytometry.
• For HIV, responsible for making/characterizing and supplying enzyme stocks to share with the lab and rate experiments for drug analogs. Protein growth, harvest, purification and quantification.
• Responsible for receiving and tracking orders, ensuring lab is in compliance with UPMC chemical and radioactive waste disposal and safety.
• UV/VIS, Total Count Infectivity Dose (TCID), Biotek.

Pittsburgh, PA, US

• Taught recitation and lab and posted grades in LIMS. Tutored students.
• Chromatography and 2-D thin-layer chromatography for the separation and purification of fatty acids. Transesterification and esterification of fatty acids from S. cerevisiae grown at different tempertures with.
• Worked at The University of Pittsburgh while completing the data and presentation to finish with a Master's in Chemistry in 2011.
• Method development, organic extractions and solid-phase extraction (SPE).
• UV/VIS spectrophotometry, high-performance liquid chromatography (HPLC), Time-of-flight (TOF), Thin Layer Chromatography (TLC), GCMS.

Baton Rouge, LA, US

• Tissue culture (HEK293), adenovirus infection, harvesting and purification.
• While working full-time, finished BA in Chemistry in December 2004.
• After December 2004, returned (part-time) to Thionville to consult and get the operations back to profitability.
• Tested endotoxins, quantifying with Real-Time Polymerase Chain Reaction (RTPCR), UV/VIS, plates, infectivity.

• Extracted DNA of samples received for genetically modified organism (GMO) for qualitative and quantitative PCR.
• Method development/problem solving and ordering supplies.
• Started methods for E. coli and Salmonella testing to increase profits and decrease turn-around time.
• Found new primer/probe pairs to increase services and participated in USDA round-robin trials for GMO.
• During this time, returned to school part-time while working full-time to finish a BA in Chemistry in 2004.
• UV/VIS, DNA standards for RTPCR.

• Help design experiments and solve problems through literature searches.
• Initial experiment was measuring the relationship of ultraviolet light exposure to the expression of malonyl dialdehyde.
• Research was also burn experiments comparing the rate of healing with different topical ointments (peptides) using rat stock.

• Extracted DNA using various methods; organic, Qiagen columns.
• Qualitative PCR and quantitative PCR.

Washington, DC, US

• Performed analytical chemistry on the aromas of food products.
• Hewlett Packard GC and a Tekmar Cryofocus purged rice samples and correlated/identified smells of certain peaks with a cone and integrator.
• Texture analysis of rice products. Overall pursuit was incorporated in the Golden Rice project.
• Performed some SPME on catfish to measure off-flavors.

Master'S, Chemistry

Bachelor'S, Chemistry

Bachelor Of Science (Bs), Biological Sciences