Ryan Murnane Email & Phone Number

San Francisco, Ca, Us

San Francisco, Ca, Us

Berkeley, California, Us

• Editing non-model bacteria strains for improvement in biomanufacturing using plasmid cloning. • Constructing new plasmids via PCR and Gibson Assembly followed by strain transformation, selection, and plasmid sequence verification after mini/midiprep. • Designing and performing PCR reactions for desired sequence detection in strains• Successfully isolated dozens of strains from a variety of environmental sources for further evaluation through successive streaking and cultures under varied parameters• Extracting DNA and RNA with further RNA depletions for Bioanalyzer analysis and NGS library preparation (Illumina/Nanopore)•Preparing liquid and plate media including custom formulas for different species

San Francisco, California, Us

Working with the Ashworth Lab in cancer research to develop an antibody-based therapeutic by screening and optimizing specific antibody fragments picked from a bacteriophage display library•Screening antibody fragments on target XCT peptide through ELISA screening•Handling bacteria cultures for producing antibodies for screening•Performing plasmid cloning and subcloning involving plasmid purification, restriction digestion, vector ligation, and bacteria transformation•Verifying genetics through PCR•Analyzing bacterial protein production through dot blot and Western blot methods•Managing human cell culture for cellular assays

Washington, Dc, Us

Compiling and analyzing data on Parthenium argentatum (guayule) growth in an effort to help increase domestic, sustainable rubber production. Studying plant genetics using plant cell tissue culture. This position has been postponed due to current COVID restrictions in the lab facility.

• Propagated a variety of new seeds and plant starts for urban, outdoor 3.5 acre Alemany Farm• Led volunteers and helped with weeding, planting, irrigation, spraying, and harvesting• Produced materials for and helped pioneer the first plant sale

San Francisco, Us

• Spearheaded and implemented GIS mapping for the program to track work sites• Programmed and taught the Youth Stewardship Program sponsored by the SCA and AmeriCorps providing outdoor, educational field trips for hundreds of youth throughout the school year.• Orchestrated over 100 volunteer habitat restoration projects involving weeding, planting, landscaping• Collaborated with numerous city gardeners to identify and create volunteer projects

• Developed knowledge of greenhouse plant propagation including techniques and substrates• Executed greenhouse maintenance like weeding and transplanting• Succeeded in propagating Miumulus aurantiacus from seed for addition to greenhouse collection

San Francisco, California, Us

Developed precise and detailed sterile lab technique in cancer cell culture.Collected and compiled experimental data assessing the effectiveness of drug and radiation treatments.Independently performed PCR, qPCR, and flow cytometry analysis in various experiments.

Bachelor Of Science - Bs, Biology/Biological Sciences, General

Certificate, Data Analytics, Computer Science

High School Diploma