Ryan Stoner Email & Phone Number

La Jolla, CA

Bethesda, MD

• Postbac in Anirban Banerjee's lab studying the biochemical and structural properties of proteins involved in heme homeostasis.
• Devised purification strategies for several difficult to purify membrane proteins.
• Extensively used PCR techniques to generate various protein expression vectors.
• Used mammalian (HEK293T) and insect cell culture (SF9 Baculovirus system) to express proteins of interest.
• Utilized yeast and bacterial cell culture to grow large quantities of membrane protein for structural studies.
• Designed a liposome-based, in-vitro heme trafficking assay.

Boulder, CO

• Undergrad in Vignesh Kasinath's lab probing the interactions of chromatin modifiers with their nucleosome substrate. Honors Thesis: Designing Chromatin Substrates for Structural and Functional Analysis of Regulatory.
• Mentored undergraduate students who joined the lab, training them in basic lab techniques.
• Performed PCR for nucleosome DNA sequence generation, molecular cloning, and site-directed mutagenesis.
• Expressed histones, PRC1, and ubiquitin in bacterial cell culture.
• Performed affinity, ion-exchange, and size-exclusion chromatography of proteins on AKTA FPLC.
• Analyzed proteins and DNA using SDS-PAGE and agarose gel electrophoresis.

Doctor Of Philosophy (Ph.D.), Biological Sciences

Bachelor Of Arts (B.A.), Biochemistry

● Graduated summa cum laude with distinction ● Certificate in Public Health