Ryan Willingham Email & Phone Number

Durham, Nc, Us

Durham, North Carolina, Us

Sapere Bio has developed a series of products based on immune aging biomarkers, including p16. Our aim is to change the way aging is measured and utilized in clinical decision-making.

Durham, North Carolina, Us

Extensive experience designing, executing, and evaluating biological assays for preclinical development, drug discovery, and product development. Comprehensive knowledge of human primary cell culture, skin biology, and inflammation. Demonstrable expertise of molecular, biochemical, and mammalian cell culture techniques. Skilled communicator, able to prepare and deliver effective presentations and lectures Successful leader, mentor, and collaborator.

Durham, North Carolina, Us

Developed a complete reconstructed human epidermal (RHE) model in order to add a new, highly demanded service to Zen-Bio’s portfolio. This RHE model will be used for ZenBio contract assays and will also be available for retail. Because one of ZenBio's many specialties is primary cell isolation, we will be able to provide RHEs to our clients at a reduced cost. Optimized the process of isolating RNA from human skin samples using the Precellys Evolution and specific bead kits. This process yields very high quality and quantity RNA as well as provides high-throughput / minimal contamination compared to previous processes. We now have increased capability to offer larger scale RNA isolation from skin to our clientsInvestigated the effects of novel ZenBio exosomes on ex-vivo skin, reconstructed human epidermis, and keratinocytes using the Asahi Max-303 UV Solar Simulator. Routinely perform contract assays for multiple ZenBio clients

Brentford, Middlesex, Gb

• Developed RHE model studies by integrating published protocols and seeking in house expertise, to analyze the effects of UV exposure in the presence and absence of Stiefel Cx productso Utilized model to evaluate biological activities of several consumer health product additives which were critical for the decision of future compound formulations• Optimized MelanoDerm models, for assessing the effect of UVB on skin pigmentation and for evaluating photoprotective and activities of SUNMAX SPF50+ and the depigmenting activities of antioxidants o Implemented the use of Chroma Meter equipment and software as well as ImagePro software in order to quantify pigmentation changes in MelanoDerm samples facilitating our interpretation of results• Worked across multidisciplinary teams including NPR/NPD, OI, and CSB in order to promptly develop and screen novel molecules and formulations which provided scientific claim support for the new products• Improved tissue embedding process which reduced effort and cost by 50% • Ensured compliance with EHS regulations by acting as the EHS liaison for CSB (2013-2015), providing insight for the site planning for the new smart labs in Upper Providence and transitioning the role of EHS representative

Rtp, Nc, Us

• Conducted scientific claims development and support use in vivo pre-clinical animal models, ex vivo human skin and in vitro experiments• Acted as liaison and coordinator between biology function of the CSB (microbiology) and product development chemists to facilitate creation of a superior next generation product • Initiated work on a novel spheroplast assay for screening of a high molecular weight encoded chemical library• Engaged with external collaborators to test compounds in novel systems• Perform SITL (secondarily infected traumatic lesion) model in mice including prep, aseptic surgery, administration of anesthesia and analgesia, dosing, and necropsy• Managed bacterial colony including necessary culture, propagation and quality control • Performed administrative responsibilities including; coordinating equipment purchase, placement, and monitoring in a new laboratory, order supplies through designated company ordering systems as well as through outside vendors

Rtp, Nc, Us

Arlington, Virginia, Us

-Assisted in the implementation of medical standard operating procedures for the 267th Military Police Company-Ensured the medical readiness of soldiers -Performed sick call procedures -Assisted with operation orders-Taught Combat Life Saver Classes

Arlington, Virginia, Us

-Triaged patients upon arrival to emergency room-Performed procedures according to orders including IVs and sutures-Maintained adequate supply inventory -Transported patients to adjacent departments (Radiology, ICU, Med-Surg)-Assisted clerk in answering phone calls regarding patient questions-Performed data entry into CHCS and CHCS II-Managed all medical supply operations for battalion-Performed sick call duties for a battalion sized unit (~1000 soldiers) on a daily basis-Performed procedures according to orders including IVs and sutures-Assisted MD and PA in minor surgical procedures-Acted as assistant medical supply sergeant and maintained accountability of medical supplies-Maintained medical platoon vehicular readiness-Assisted in the development of operating procedures while conducting field training-Performed CPR in cardiac emergencies-Conducted convoy operations and the primary medical support personnel