Simone Giuseppe Giuffrida Email & Phone Number

United Kingdom

Cardiff, Wales, United Kingdom

Synthesis and downstream process of modified oligonucleotide therapeutics.- Use of the MerMade 12 synthesiser to synthesise hybrid DNA/modified RNA oligonucleotides;- Cleavage from different supports and deprotection of synthesised oligonucleotides;- Purification via anion exchange and/or ion pairing RP preparative HPLCs;- Desalting of the oligonucleotides;- Crude and final purities control on the LC-MS;- Annealing of sense and antisense strands to generate either… Show more Synthesis and downstream process of modified oligonucleotide therapeutics.- Use of the MerMade 12 synthesiser to synthesise hybrid DNA/modified RNA oligonucleotides;- Cleavage from different supports and deprotection of synthesised oligonucleotides;- Purification via anion exchange and/or ion pairing RP preparative HPLCs;- Desalting of the oligonucleotides;- Crude and final purities control on the LC-MS;- Annealing of sense and antisense strands to generate either mono- and dualgenes. Show less

Wrocław, Dolnośląskie, Poland

During my postdoctoral position at the PORT, the design, synthesis and characterisation of abiotic sequence-defined oligourethanes have been investigated for their 3D structures, controlled by both the sequence of monomers and chirality. NMR and CD spectroscopies have been employed to understand and study the behaviour of the synthesised oligourethanes in different solvent systems. The potential folding of such oligomers in different solvent systems can be exploited for different applications… Show more During my postdoctoral position at the PORT, the design, synthesis and characterisation of abiotic sequence-defined oligourethanes have been investigated for their 3D structures, controlled by both the sequence of monomers and chirality. NMR and CD spectroscopies have been employed to understand and study the behaviour of the synthesised oligourethanes in different solvent systems. The potential folding of such oligomers in different solvent systems can be exploited for different applications in several fields of research such as Material Science, Electronics and Life Science. - Supervisor: Dr. R. Szweda Show less

Bath, Regno Unito

During the PhD experience in Prof Pascu's group, different biosensors, either optical or electrochemical, were designed, synthesised and characterised. The fluorescent systems were investigated in different living cancerous cells via fluorescence microscopies to assess their localisation and interactions within cells. A potential electrochemical biosensor, consisting of prostate cancer targeting-peptide, was designed and built which was able to discriminate prostate cancer cells from other cell… Show more During the PhD experience in Prof Pascu's group, different biosensors, either optical or electrochemical, were designed, synthesised and characterised. The fluorescent systems were investigated in different living cancerous cells via fluorescence microscopies to assess their localisation and interactions within cells. A potential electrochemical biosensor, consisting of prostate cancer targeting-peptide, was designed and built which was able to discriminate prostate cancer cells from other cell lines.Title of Thesis: Development of new peptide conjugates as biosensors and imaging tools and investigations into their interactions with prostate cancer cells- Topics covered: Design, synthesis and characterization of fluorescent compounds based on naphathalenediimides and exploration in the cellular environment.- Supervisor: Prof. S. Pascu; co-supervisor: Dr. P. Estrela Show less

Catania, Italy

During the internship at the Institute of Biomolecular Chemistry of CNR in Catania, I gained experience in the design, synthesis and characterization of biomimetic compounds based on calix[4]arenes. Such architecture provided a prearranged scaffold for the conjugation of individual units of a-D-Mannose or oligomannosides on the upper rim of calix[4]arenes. - Thesis: Preliminary studies for obtaining biomimetic compounds useful for the development of an anti-HIV vaccine.- Topics… Show more During the internship at the Institute of Biomolecular Chemistry of CNR in Catania, I gained experience in the design, synthesis and characterization of biomimetic compounds based on calix[4]arenes. Such architecture provided a prearranged scaffold for the conjugation of individual units of a-D-Mannose or oligomannosides on the upper rim of calix[4]arenes. - Thesis: Preliminary studies for obtaining biomimetic compounds useful for the development of an anti-HIV vaccine.- Topics covered: Design, synthesis and characterization of biomimetic compounds based on calix[4]arenes presented individual units of a-D-mannose or oligomannosides on the upper rims.- Supervisor: Prof. S. Sciuto; Co-supervisor: Dr. C. Geraci Show less

Catania, Italia

During the stage at Prof Tomaselli's research laboratory, fluorescent compounds based on bis-thiocarbazones were designed and synthesised. Such fluorescent ligands were able to selectively bond Cu(II) ions which were studied to develop intracellular sensors for the detection of the imbalance of such ion.- Thesis: Synthesis and characterization of new ionophores for Copper.- Topics covered: Design, synthesis and characterization of fluorescent compounds based on… Show more During the stage at Prof Tomaselli's research laboratory, fluorescent compounds based on bis-thiocarbazones were designed and synthesised. Such fluorescent ligands were able to selectively bond Cu(II) ions which were studied to develop intracellular sensors for the detection of the imbalance of such ion.- Thesis: Synthesis and characterization of new ionophores for Copper.- Topics covered: Design, synthesis and characterization of fluorescent compounds based on bis-thiosemicarbazones able to recognize the Cu(II).- Supervisor: Prof. G. Tomaselli Show less

Doctor Of Philosophy - Phd, Chemistry

Master'S Degree, Organic And Bioorganic Chemistry, 110 E Lode / 110

Bachelor'S Degree, Chemistry, 103 / 110