Saadman Islam Email & Phone Number

Brentford, Middlesex, GB

• Focus on the development and execution of downstream processes in different research and development contexts (early development, processability, process development, process understanding, troubleshooting, Life cycle).
• Develop, optimize, and scale-up downstream protein purification processes.
• Execute large scale purifications using depth filtration, centrifugation, chromatography and tangential flow filtration (TFF).
• Characterize proteins using SDS-PAGE, BCA assay, and other biochemical assays to determine protein purity and quality attributes.
• Work in close collaboration with experts in upstream, downstream and various analytical technologies
• Write batch records for fermentation and expression protocols.

Cambridge, Massachusetts, US

• Developed and optimized scalable downstream processes for the separation and purification of virus particles using preparative SEC resins, analytical HPLC, and testing various run conditions such as flow velocity, %CV.
• Utilized depth filtration, ultrafiltration, and diafiltration to purify, concentrate and buffer exchange virus particles.
• Experienced in running PicoGreen & BCA assays using a Tecan liquid handler.
• Analyzed drug final formulations using techniques such as Differential Scanning Fluorometry, Dynamic Light Scattering & Static Light Scattering to evaluate their thermal stability & physical properties.
• Led monthly department meetings & collaborated with operations to manage inventory & instrument acquisition.
• Created SOPs for tech-transfers.

Cambridge, Massachusetts, US

• Optimized processes for mammalian cell culture, including passage, chemical transfection, and lysis.
• Utilized DoE & JMP software to analyze data and improve protein expression & cell viability.
• Managed equipment procurement & vendor collaborations.
• Experienced in operating cell analyzer instruments for cell counting.
• Volunteered on-boarding of new teammates.

Cambridge, Massachusetts, US

• Responsible for viral particle expression, purification, construct screening, and protein characterization. My work contributed to the first-ever published EM structure of an Anellovirus. Key Responsibilities include:
• Perform protein and viral particle purification procedures using standard chromatography techniques and equipment (e.g. AKTA FPLCs).
• Perform analytical procedures such as SDS-PAGE, and western blotting.
• Work with mammalian/insect cell culture procedures, including transfections, cell passaging, thawing, and freezing of cell stocks.
• Participate in experiment design and data analysis.
• Collaboration with multi-disciplinary teams.

Worcester, Massachusetts, US

Performed novel kinetic trials via Circular Dichroism measurements of Insulin Degrading Enzyme (IDE) on various substrates with and without the presence of potential polyphenol inhibitors to determine Michaelis-Menten constants Km & kcat. Performed SDM on IDE active-site, expressed and purified the mutant protein, and performed various biophysical.

Worcester, Massachusetts, US

Awarded the Beaver Fellowship for Brain Biochemistry research. I enrolled in doing the research-oriented M.S. program with Prof. Noel Lazo in this lab. We are constantly collaborating with Prof. Don Spratt's lab for cell cultures, primer vector design, bacterial protein expression, and purification using the AKTA Start/Pure FPLC.

Worcester, Massachusetts, US

Provided paraprofessional advising to undergraduate students in two distinct residential environments. Developed and conducted programs on diversity, chemical abuse, personal development, relationships, security, and academic performance. Managed administrative tasks including room condition reports, maintenance requests, incident reports, nightly duty.

Worcester, Massachusetts, US

Worcester, Massachusetts, US

Synthesis of oligopeptides to potentially inhibit enzymes promoting cancer metastasis, and testing Bioassays. Conducted many peptide synthesis reactions, purification by column chromatography, NMR and LC-MS analysis.

Worcester, Massachusetts, US

Awarded the SURE grant. Aided Ph.D. students and faculty in measuring the maternal stress response, by expression of cortisol, of three-spine stickleback, Gasterosteus aculeatus, and its effects on developing offspring. Performed routine tests and collected data from the adult fish and fry fish species via digital photographs (to track growth and take.

Downstream Processing

Master Of Science - Ms, Biochemistry And Molecular Biology

Bachelor'S Degree, Biochemistry And Molecular Biology (Bcmb)

Cambridge International Examinations, A-Levels, Igcse/O-Levels