Customer Applications Coordinator for G4 Sequencing Platform: Technical lead for customer collaborations, focusing on R&D efforts to demonstrate the G4's capability to sequence samples across various applications (WGS, WES, Methyl-Seq, microbial, RNA-Seq, low diversity etc.Co-lead of Applications in situ team for assay development on the G4X. Responsible for protocol, process development, and training.G4X in situ platform Customer Services Technical Lead: coorindate R&D efforts for sample intake, processing, optimizations and data delivery.

NGS Sequencing Applications Scientist:Leadership: Led a team of 12 on multiple projects.Library Preparation: Oversaw in-house library preparation, including the testing of commercial kits, designing novel adapters/blockers, and the manufacturing/validation of commercial libraries.Innovation: Developed novel library preparation methods and other sequencing applications for the Singular Genomics G4 sequencing platform.HD-Seq: Served as both project lead and individual contributor for a novel library preparation and sequencing method, achieving higher accuracies (>Q50) at lower throughput.Customer Applications Coordinator: Acted as the technical lead for customer collaborations, focusing on R&D efforts to demonstrate the G4's capability to sequence samples across various applications (WGS, WES, Methyl-Seq, microbial, RNA-Seq, low diversity etc.

Responsible for Research and Development of molecular biology based products for ligation, PCR, NGS and in vitro transcription assays. Experience in developing novel ideas and applying chemistry-based solutions (modified primers and nucleotides) to improve assay specificity and sensitivity. • Developed a small RNA library preparation kit (CleanTag™) using chemically modified adapters which reduce adapter dimer to allow for single-cell input detection and elimination of a once critical gel purification step. • Developed several PCR and RT-PCR Master Mixes utilizing Hot Start dNTPs (CleanAmp™ product line)• Development of GC-rich PCR Master Mix for improved amplification of GC-rich sequences and downstream Sanger Sequencing. • Development of several standardized pathogen detection assays (multiplex qPCR and qRT-PCR) to diagnose infectious AGE (acute gastroenteritis). • Assisted with implementing CleanAmp™ Hot Start dNTPs into mtDNA amplification strategies for Forensic use and performed QC of mitoPrimers™ product line for amplification of mtDNA and downstream Sanger sequencing• Optimization of in vitro transcription reactions and subsequent purification steps• In vitro transcription with modified NTPs and codon optimization of mRNA transcripts• Experience with NGS library preparation methods: small RNA, RNA, and DNA• Experience with NGS data analysis programs: FileZilla, Integrative Genomics Viewer (IGV), UCSC Genome Browser, Geneious, BaseSpace, Galaxy.

Researched caveolae specific tumor targets for site directed treatment of various cancers. 1.Project co-coordinator for Mass Spectrometry data validation: Responsible for validating Mass Spectometry data generated from rat lung and heart endothelial cells, specifically proteins in plasma membrane and caveolae fractions. Techniques used: western blots, immunoprecipitations, blood cell separation, immunoflourescence, Elisas, commercial and custom antibody research and testing, data input, colloidal and silver staining. Organized and catalogued the results, as well as those obtained from senior scientists, for input to our in-house database.2.Molecular cloning and protein expression of mouse albumin in preparation of creating an abraxane-type drug3.Construction of a lentiviral vector with cav-1 siRNA and expression for gene knockdown in mice4.Preparation of SV40 stocks

Undergraduate Thesis Research: GENETIC AND PHENOTYPIC ANALYSIS OF TEMPERATURE SENSITIVE MORPHOLOGICAL MUTANTS IN CAENORHABDITIS ELEGANS*Successfully mapped a novel gene in C. elegans, that functions in development, to a small region on chromosome II. *Defined critical period in C. elegans development for that gene using reciprocal temperature shift experiments*Created 4D movies of developing embryos in mutant worms*Completed detailed phenotypic analysis of a temperature sensitive mutant*Techniques Used: Bulk Segregant snip-SNP mapping, PCR , Restriction digests, DNA extraction, slide preparation of embryos, genomic preps from C. elegans, phenol-chloroform extractions, two and three factor mapping,